Artery Research

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Volume 7, Issue 3-4, September 2013, Pages 211 - 215

Absence of Helicobacter pylori infection in coronary atherosclerosis disease in Northeast of Iran

Authors
Mohammad Hadi Sadeghiana, Seyed Abbas Tabatabaee Yazdia, Hossein Ayatollahia, *, Kiarash Ghazvinib, Mohammad Reza Keramatia, Ehsan Ghayoor Karimianic, Maryam Sheikhic, Somaye Vakili Ahrarirodid, Ghohar Shaghayeghc
aCancer Molecular Pathology Research Centre, Ghaem Medical Centre, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
bDepartment of Microbiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
cCancer Molecular Pathology Research Centre, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
dFaculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
*Corresponding author. Tel.: +98 5118012584. E-mail address: ayatollahih@mums.ac.ir (H. Ayatollahi).
Corresponding Author
Hossein Ayatollahi
Received 22 May 2013, Revised 13 August 2013, Accepted 20 August 2013, Available Online 12 September 2013.
DOI
10.1016/j.artres.2013.08.003How to use a DOI?
Keywords
Atherosclerosis; Coronary arteries; Helicobacter pylori; Polymerase chain reaction
Abstract

Background: Atherosclerosis is an inflammatory disorder in which infectious agents are incriminated as contributors. It is suggested that Helicobacter pylori may be a risk factor of atherosclerosis.

Methods: This study was performed whether there are differences between coronary artery with and without atherosclerotic plaque in the presences of H. pylori DNA using polymerase chain reaction (PCR) method.

This case-control study carried out on formalin-fixed paraffin-embedded (FFPE) of coronary artery biopsies. These specimens were obtained from 30 patients with coronary atherosclerosis and 30 subjects without atherosclerosis. Multiple sections were cut from each FFPE, the Deoxyribonucleic acid (DNA) was extracted by non-heating extraction method, then PCR was carried out for detection of H. pylori DNA and finally its products were analyzed by electrophoresis.

Results: The age range in the patient’s group was from 18 to 50 years and the male to female ratio was 5 to 1. None of the coronary tissue samples had positive PCR results for H. pylori DNA and all of the control samples were also negative.

Conclusions: This study showed that H. pylori infection is not associated with coronary artery atherosclerosis.

Copyright
© 2013 Published by Elsevier B.V. on behalf of Association for Research into Arterial Structure and Physiology.
Open Access
This is an open access article distributed under the CC BY-NC license.

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<Previous Article In Issue
Next Article In Issue>
Journal
Artery Research
Volume-Issue
7 - 3-4
Pages
211 - 215
Publication Date
2013/09/12
ISSN (Online)
1876-4401
ISSN (Print)
1872-9312
DOI
10.1016/j.artres.2013.08.003How to use a DOI?
Copyright
© 2013 Published by Elsevier B.V. on behalf of Association for Research into Arterial Structure and Physiology.
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

risenwbib
TY  - JOUR
AU  - Mohammad Hadi Sadeghian
AU  - Seyed Abbas Tabatabaee Yazdi
AU  - Hossein Ayatollahi
AU  - Kiarash Ghazvini
AU  - Mohammad Reza Keramati
AU  - Ehsan Ghayoor Karimiani
AU  - Maryam Sheikhi
AU  - Somaye Vakili Ahrarirodi
AU  - Ghohar Shaghayegh
PY  - 2013
DA  - 2013/09/12
TI  - Absence of Helicobacter pylori infection in coronary atherosclerosis disease in Northeast of Iran
JO  - Artery Research
SP  - 211
EP  - 215
VL  - 7
IS  - 3-4
SN  - 1876-4401
UR  - https://doi.org/10.1016/j.artres.2013.08.003
DO  - 10.1016/j.artres.2013.08.003
ID  - Sadeghian2013
ER  -