Elevated levels of IL-6 and IL-9 in the sera of patients with AAA do not correspond to their production by peripheral blood mononuclear cells

Hamid Aria; Mehdi Kalani; Hossein Hodjati; Mehrnoosh Doroudchi

doi:10.1016/j.artres.2017.12.007

<Previous Article In Issue

Next Article In Issue>

Volume 21, Issue C, March 2018, Pages 43 - 52

Elevated levels of IL-6 and IL-9 in the sera of patients with AAA do not correspond to their production by peripheral blood mononuclear cells

Authors

Hamid Aria^a, Mehdi Kalani^b, Hossein Hodjati^c, Mehrnoosh Doroudchi^a^{, *}

^aDepartment of Immunology, School of Medicine, Shiraz University of Medical Sciences, Iran

^bDepartment of Immunology, Professor Alborzi Clinical Microbiology Research Center, Nemazee Hospital, Shiraz University of Medical Sciences, Shiraz, Iran

^cDepartment of Vascular Surgery, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

^*Corresponding author. Memory T cell Laboratory, Department of Immunology, School of Medicine, Shiraz University of Medical Science, P.O. Box: 71345-3119, 71348-45794, Shiraz, Iran. Fax: +98 71 32351575. E-mail address: mdoroud@sumc.ac.ir (M. Doroudchi).

Corresponding Author

Mehrnoosh Doroudchi

Received 2 October 2017, Revised 24 December 2017, Accepted 30 December 2017, Available Online 11 January 2018.

DOI: 10.1016/j.artres.2017.12.007 How to use a DOI?
Keywords: Abdominal Aortic Aneurysm (AAA); IL-9; IL-10; PBMC; Serum
Abstract: Background: Abdominal Aortic Aneurysm (AAA) is the stable local dilatation of abdominal aorta. AAA is an inflammatory condition in which cytokines may play a pathogenic role.

Methods: Peripheral Blood Mononuclear cells (PBMCs) were isolated from 5 men, with confirmed diagnosis of AAA and aortic dilation greater than 5.5 cm, and 5 men with normal/insignificant angiography, CT-Scan and Ultrasonography results. The supernatant of PBMCs, rested overnight in RPMI containing 10%-FBS, removed to measure IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-13, IL-17A, IL-17F, IL-21, IL-22, IFN-γ and TNF-α using a commercial fluorescent-labeled bead assay.

Results: The mean serum IL-6 and IL-9 levels were significantly higher in patients than controls (P = 0.007 and P = 0.007, respectively). PBMCs from patients produced lower levels of IL-6 and IL-9 compared to controls but the differences were not significant. While serum TNF-α level was not different between groups, its production by PBMCs of patients was significantly lower than controls (P = 0.047). The mean serum levels of IL-10 and IFN-γ in patients were marginally higher than controls (P = 0.055, P = 0.055, respectively). Mean serum IL-2 level was not different between the groups but its production by PBMCs of patients was significantly higher than the control group (P = 0.047).

Conclusions: Our study showed alteration in the levels of cytokines from inflammatory, Th1, Th2 and Th17 subtypes in the sera of patients with AAA. The production of IL-6, IL-9, IFN-γ and IL-10, however, was not solely attributed to the PBMCs. Therefore, participation of other cells in the tissue or blood should be considered in their production.
Open Access: This is an open access article distributed under the CC BY-NC license.

Download article (PDF)
View full text (HTML)

<Previous Article In Issue

Next Article In Issue>

Journal: Artery Research
Volume-Issue: 21 - C
Pages: 43 - 52
Publication Date: 2018/01/11
ISSN (Online): 1876-4401
ISSN (Print): 1872-9312
DOI: 10.1016/j.artres.2017.12.007 How to use a DOI?
Open Access: This is an open access article distributed under the CC BY-NC license.

Cite this article

ris enw bib

TY  - JOUR
AU  - Hamid Aria
AU  - Mehdi Kalani
AU  - Hossein Hodjati
AU  - Mehrnoosh Doroudchi
PY  - 2018
DA  - 2018/01/11
TI  - Elevated levels of IL-6 and IL-9 in the sera of patients with AAA do not correspond to their production by peripheral blood mononuclear cells
JO  - Artery Research
SP  - 43
EP  - 52
VL  - 21
IS  - C
SN  - 1876-4401
UR  - https://doi.org/10.1016/j.artres.2017.12.007
DO  - 10.1016/j.artres.2017.12.007
ID  - Aria2018
ER  -

download .riscopy to clipboard