Rapid Identification of Three Waterborne Pathogens in Water Samples by Multiplex Touchdown PCR

DOI

10.2991/mse-15.2016.33How to use a DOI?

Keywords

vibrio parahemolyticus, yersinia enterocolitica, shigella, multiplex touchdown PCR

Abstract

The purpose of this study was to develop a multiplex touchdown PCR to identify three waterborne pathogens in water samples. Specific primers based on VP1332, inv and ipaH gene sequences were designed for V. parahaemolyticus, Y. enterocolitca and Shigella, respectively. By optimizing reaction conditions, assays obtained high specifity, sensitivity and repeatability. The detection limit can be as low as 0.005ng/" "l V. parahaemolyticus and 0.5ng/" "l Y. enterocolitca and 0.005ng/" "l of Shigella respectively. The detection process was about 3~4 hours, saving a lot of time compared with microbiological culture method that required 2~3 days. The system could be useful for the detection of multiple pathogens in water samples to protect public health.

Copyright

© 2016, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Yanjiao Hou
AU  - Guanlin Li
AU  - Chen Shao
AU  - Shihe Shao
PY  - 2016/03
DA  - 2016/03
TI  - Rapid Identification of Three Waterborne Pathogens in Water Samples by Multiplex Touchdown PCR
BT  - Proceedings of the 2015 International Conference on Mechanical Science and Engineering
PB  - Atlantis Press
SP  - 185
EP  - 190
SN  - 2352-5401
UR  - https://doi.org/10.2991/mse-15.2016.33
DO  - 10.2991/mse-15.2016.33
ID  - Hou2016/03
ER  -