Activity of Ethanol-derived Fraction of Clove Leaves and Eugenol Compound as Antiaging Agent in the Yeast Model Organism Schizosaccharomyces pombe

DOI

10.2991/absr.k.210621.086How to use a DOI?

Keywords

antiaging, clove leaves, eugenol, Schizosaccharomyces pombe, oxidative stress

Abstract

Clove is known to have high antioxidant and antivirulence activity. The antioxidant activity of clove extract presumably correlates with the extension of cellular life span (antiaging activity). Crude extract of clove leaves has been reported to prolong the life span of yeast cells, however lack of information available regarding antiaging activity of its corresponding ethanol fraction and eugenol. Eugenol is the major phenolic compound found in clove leaves. Thus, the aim of this study was to analyze the mode of action of ethanol fraction of clove leaves and eugenol at the cellular and molecular levels in prolonging life span of the yeast model organism Schizosaccharomyces pombe. The results showed that the ethanol fraction of clove leaves and eugenol compounds could prolong the yeast life span at the lowest concentration, 25 ppm and 10 ppm, respectively. The test results also showed that the ethanol fraction of clove leaves and eugenol compounds can induce yeast tolerance against oxidative stress (1mM, 2mM, and 3mM H2O2). Our docking molecular revealed that eugenol compounds can bind to regulator of oxidative stress response pathway, sty1 and ctt1 proteins but not on the active side. Further study is required to clarify the effect of eugenol toward the particular stress response pathway. Taken together, our study suggests the potential antiaging activity of ethanol fraction of clove leaves and eugenol compounds.

Copyright

© 2021, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Download article (PDF)

TY  - CONF
AU  - Sity Azizah Anwar
AU  - Rika Indri Astuti
AU  - Dedy Duryadi Solihin
PY  - 2021
DA  - 2021/06/23
TI  - Activity of Ethanol-derived Fraction of Clove Leaves and Eugenol Compound as Antiaging Agent in the Yeast Model Organism Schizosaccharomyces pombe
BT  - Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)
PB  - Atlantis Press
SP  - 515
EP  - 522
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.210621.086
DO  - 10.2991/absr.k.210621.086
ID  - Anwar2021
ER  -