Effect of Skim Milk on Sperm Motility of Brek Fish, Systomus orphoides (Valenciennes, 1842) for Short Term Preservation
- DOI
- 10.2991/absr.k.210621.050How to use a DOI?
- Keywords
- motility, preservation, skim milk, sperm, Systomus orphoides
- Abstract
The purpose of this research was to evaluate the effect of skim milk on sperm motility of Brek fish, Systomus orphoides (Valenciennes, 1842) after 48 hours in 4°C. The method in this research is sperm was collected by hand stripping and was diluted by diluent, which consisted of the fish ringer, skim milk and methanol 10%. The ratio of sperm to diluent is 1:9. The concentrations of skim milk used in this research were 0%, 5%, 10%, 15%, and 20%, respectively. The sperm was stored at 4 °C for 48 hours after diluted sperm is thawed in room temperate for 1 minute and sperm motility was observed in improved Neubauer with a light microscope.The motility percentage was analyzed using ANOVA and Tukey test. According to the Annova test (P>0.05), 10% of skim milk showed the highest sperm motility (92.18 ± 3.05) % after five replication per dose. Ten percent of skim milk was the optimum concentration to preserve sperm motility at 4°C for 48 hours compared to 0%, 5%, 15%, and 20%. The result of each concentration is 90,85%, 84,68%, 79,54%, and 74,14% respectively.
- Copyright
- © 2021, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Rifqi Ramadhana AU - Abinawanto AU - Rudhy Gustiano PY - 2021 DA - 2021/06/23 TI - Effect of Skim Milk on Sperm Motility of Brek Fish, Systomus orphoides (Valenciennes, 1842) for Short Term Preservation BT - Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020) PB - Atlantis Press SP - 296 EP - 302 SN - 2468-5747 UR - https://doi.org/10.2991/absr.k.210621.050 DO - 10.2991/absr.k.210621.050 ID - Ramadhana2021 ER -