Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)

The Role of PDGF Gene in Tissue Regeneration of Digit Tip Mice (Mus musculus)

Authors
Titta Novianti, Tazkia Ayu Safitri, Febriana Dwi Wahyuni, It Jamilah, Syafruddin Ilyas
Corresponding Author
Syafruddin Ilyas
Available Online 23 June 2021.
DOI
10.2991/absr.k.210621.071How to use a DOI?
Keywords
PDGF, tissue regeneration, mice, granule phase, amputated
Abstract

Some genes play a role in the tissue regeneration process and they have specific functions. One of them is the platelet-derived growth factors (PDGF) gene that have a role in the wound healing process because it induces cell mitosis and thereby causes cell proliferation. PDGF protein expression is thought to chemotactically recruit many cell types including smooth muscle, fibroblasts, and monocytes to migrate to the wound site. These research aims are to analyze the role of PDGF gene expression in the process of digit tip mice tissue regeneration. This study used 24 mice (Mus musculus) were amputated at the digit tip and the growth was observed on days 0, 1, 3, 5, 10, 15, and 25. We isolated RNA from each tissue after amputation on days 0, 1, 3, 5, 10, 15, and 25 and analyzed the RNA expression of the PDGF gene using the qPCR method. PDGF gene expression reached its peak on day 10 on the granule phase and decreased until day 25. The peak expression of the PDGF gene indicates the role of the gene in the tissue regeneration of digit tip mice. The normality test for PDGF gene expression was normally distributed (p> 0, 05) and the variation test between each treatment was different significantly (p <0.05).

Copyright
© 2021, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)
Series
Advances in Biological Sciences Research
Publication Date
23 June 2021
ISBN
978-94-6239-399-8
ISSN
2468-5747
DOI
10.2991/absr.k.210621.071How to use a DOI?
Copyright
© 2021, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Titta Novianti
AU  - Tazkia Ayu Safitri
AU  - Febriana Dwi Wahyuni
AU  - It Jamilah
AU  - Syafruddin Ilyas
PY  - 2021
DA  - 2021/06/23
TI  - The Role of PDGF Gene in Tissue Regeneration of Digit Tip Mice (Mus musculus)
BT  - Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)
PB  - Atlantis Press
SP  - 420
EP  - 425
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.210621.071
DO  - 10.2991/absr.k.210621.071
ID  - Novianti2021
ER  -