Jackfruit Seed Extract Exposure on Zebrafish Embryos as Initial Screening Model for Covid-19 Treatment

DOI

10.2991/absr.k.210621.040How to use a DOI?

Keywords

ACE-2, Artocarpus heterophyllus, Zebrafish, COVID-19

Abstract

The World Health Organization establishes the coronavirus COVID-19 as a pandemic in March 2020. The receptor-binding protein in Coronavirus will bind to ACE-2 found in the heart, lungs, kidneys, and digestive tract, facilitating the entry of the virus into cells target to infect its host. High infection rates and an undiscovered COVID-19 vaccine encourage disease prevention. Several alternatives used for herbal plants have been recommended, one of which is jackfruit seed (Artocarpus heterophyllus). This study aims to screen the use of jackfruit seed extract (JSE) on the development of zebrafish embryos. This study used an experimental method with a randomized block design (RBD) and each treatment was repeated 3 times. The zebrafish were grouped into three treatment and one control groups. The concentration of jackfruit seed extract (Artocarpus heterophyllus) was 10, 7.5, 5, and 0 ug/mL for the control group, respectively. Result showed no effect of JSE on the morphological features, hatching and survival rate as well as heartbeat rate in all treatments. All embryos showed normal development as indicated by the detachment of tail – yolk sac and midbrain-hindbrain boundaries formation at 24 hpf. The heartbeat rate showed no differences between JSE treatment and control; both showed a standard heart rate between 140 – 180 bpm. Moreover, Statistical analysis conducted on the data showed no significant differences on each treatmen. In conclusion, the JSE treatment didnot caused development defect on zebrafish embryos.

Copyright

© 2021, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Download article (PDF)

TY  - CONF
AU  - Atika Ayu Kusumaningtyas
AU  - Bambang Retnoaji
PY  - 2021
DA  - 2021/06/23
TI  - Jackfruit Seed Extract Exposure on Zebrafish Embryos as Initial Screening Model for Covid-19 Treatment
BT  - Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)
PB  - Atlantis Press
SP  - 240
EP  - 245
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.210621.040
DO  - 10.2991/absr.k.210621.040
ID  - Kusumaningtyas2021
ER  -