The Study Application of Water Hyacinth Biofertilizer Towards the Existence of Endophytic Bacteria in Maize Plant
- DOI
- 10.2991/absr.k.210621.016How to use a DOI?
- Keywords
- Endophytic bacteria, Identification, Water hyacinth, B. licheniformis
- Abstract
Endophytic bacteria are bacteria that live in plant tissue without destroying the host plant. The presence of endophytic bacteria in plant tissue can produce phytohormones to stimulate growth and increase plant resistance to disease. The purpose of this study is to identify endophytic bacteria from the root tissue of maize plants treated with water hyacinth biofertilizer and to calculate the number of bacterial populations. The research was conducted at the Experimental Garden and Laboratory of the Payakumbuh State Agricultural Polytechnic. The treatments of biofertilizer were carried out by immersing the seeds in biofertilizer 4% solution for 12 hours, then maize plants were treated with a biofertilizer in the planting hole, and the age of the plants was 14, 28, 42, 56 days after planting. Observation parameters were carried out on endophytic bacteria of the maize plant and the number of bacterial populations. Identification of bacterial morphology based on colony shape, colony edges, colony elevation, surface, color, bacterial density, and gram staining. Bacterial species were identified using molecular analysis based on the 16S rRNA gene fragment. The identification results of maize root endophytic bacteria found 4 dominant bacterial with several bacterial populations species such as B. paramycoides 12,1 x 107 CFU/ml, P. aeruginosa 4,3 x 107 CFU/ml, B. subtilis 9,4 x 107 CFU/ml, and B. licheniformis 5,95 x 106 CFU/ml.
- Copyright
- © 2021, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Yun Sondang AU - Ramond Siregar AU - Khazy Anty PY - 2021 DA - 2021/06/23 TI - The Study Application of Water Hyacinth Biofertilizer Towards the Existence of Endophytic Bacteria in Maize Plant BT - Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020) PB - Atlantis Press SP - 94 EP - 100 SN - 2468-5747 UR - https://doi.org/10.2991/absr.k.210621.016 DO - 10.2991/absr.k.210621.016 ID - Sondang2021 ER -