DNA Barcode of Black Capped White Eye Zosterops atricapilla (Aves: Zosteropidae) From Bengkulu Indonesia Based on Mitochondrial DNA COI Gene
- DOI
- 10.2991/absr.k.210621.061How to use a DOI?
- Keywords
- Conservation, Cytochrome Oxidase I, illegal trafficking, mtDNA, Zosterops atricapilla
- Abstract
The study aims to determine the genetic character of Black-capped white-eye (Zosterops articapilla) using the mtDNA COI gene. Blood sample was collected from bird market in Bengkulu city and its DNA genome isolation and purification following the Protocols of Dneasy® Blood and Tissue Kit Cat no 69504 (50) based on the procedure of Spin-Column Protocol, Qiagen. We used polymerase chain reaction machine for amplification DNA template with specific primer (ZCOIF and ZCOIR). The results showed that there are nucleotide variations on five individual Black-capped white-eye with the COI gene sequence length 750 bp. We found of conservative site (C) is 743 sites, variations (V) seven sites, parsimony (Pi) four sites, singleton (S) three sites, and nucleotide base pairs of Zosterops atricapilla to adenine and thymine (AT) 55.9% and guanine and cytosine (GC) 44.1%. Fifth individual of Black-capped white-eye are separated with average intraspecies genetic distance 0.4%, while its interspecific genetic distance is 3.47% and with the outgroup 12.1%. We think, these COI gene sequences very important to quick identification and could be used as a forensic tool in preventing its trafficking.
- Copyright
- © 2021, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Jarulis AU - Ahmat Fakhri Utama AU - Sipriyadi AU - Santi Nurul Kamilah PY - 2021 DA - 2021/06/23 TI - DNA Barcode of Black Capped White Eye Zosterops atricapilla (Aves: Zosteropidae) From Bengkulu Indonesia Based on Mitochondrial DNA COI Gene BT - Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020) PB - Atlantis Press SP - 357 EP - 363 SN - 2468-5747 UR - https://doi.org/10.2991/absr.k.210621.061 DO - 10.2991/absr.k.210621.061 ID - 2021 ER -