The Use of Calcium Hydroxide with Different Soaking Time on Cassava Peel for Reducing HCN, and Its Effect on Rumen Fermentation

DOI

10.2991/absr.k.220309.054How to use a DOI?

Keywords

Cassava peel; HCN; Calcium hydroxide; Rumen characteristic

Abstract

The objective of this study is to determine the best dose of calcium hydroxide [Ca(OH)₂] and soaking time for reducing hydrogen cyanide (HCN) level of cassava peel and its effect on rumen fermentation. This study consists of two stages. The first stage was the treatment of soaking cassava peels in Ca(OH)₂ with different doses and soaking times. This study was conducted using the factorial 3x3 randomized block design (RBD) with three replications. The first factor was Ca(OH)₂ doses (0%, 0.25%, 0.5%) and the second factor was the soaking time (1, 2, 3 hours). The Parameter in the first stage was to determine the HCN content of cassava peel. The second stage of this study was an in vitro study to determine the characteristics of the rumen fluid: pH, ammonia (NH₃) and volatile fatty (VFA) conducted using in randomized block design. The result of the first stage showed that there is no interaction (P>0.05) between Ca(OH)₂ doses and soaking time on the content of hydrogen cyanide (HCN) content of cassava peels. A factor, Ca(OH)₂ dose had a significant effect (P<0.05) on HCN content with an average of 27.23 ppm - 32.31 ppm; while B factor, soaking time had a highly significant effect (P<0.01) on HCN level and nutrient content. The average HCN content ranged from 24.83 ppm - 33.51 ppm. The results of the second stage in the in vitro fermentability study on the characteristics of rumen fluid showed that the pH value of rumen fluid ranged from 6.57- 6.92, was not affected by a combination of treatment, but levels of NH₃ and VFA were highly significant different (P<0.01). The average NH₃ and VFA content ranged from 9.92 - 11.76 mg/100 ml, and 98.33 - 115 mM respectively. It can be concluded that the best dose of Ca(OH)₂ used for reducing the HCN level of cassava peels was 0.25% with a soaking time of 3 hours. The best combination was found in the A3B2 treatment. at a dose of 0.50% Ca(OH)₂ and 2 hours of soaking time. The best levels of VFA and NH3 were 115 mM VFA, 11.62 mg NH₃/100 ml respectively with rumen pH 6.88.

Copyright

© 2022 The Authors. Published by Atlantis Press International B.V.

Open Access

This is an open access article under the CC BY-NC license.

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TY  - CONF
AU  - Fauzia Agustin
AU  - Erpomen
AU  - Hanannisa Suryadi
AU  - Novirman Jamarun
PY  - 2022
DA  - 2022/03/29
TI  - The Use of Calcium Hydroxide with Different Soaking Time on Cassava Peel for Reducing HCN, and Its Effect on Rumen Fermentation
BT  - Proceedings of the International Conference on Improving Tropical Animal Production for Food Security (ITAPS 2021)
PB  - Atlantis Press
SP  - 274
EP  - 281
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220309.054
DO  - 10.2991/absr.k.220309.054
ID  - Agustin2022
ER  -