Egg Production Characteristic and the Study of Follicle-stimulating Hormone Receptor Gene on Various of Sentul Chicken

DOI

10.2991/absr.k.220309.002How to use a DOI?

Keywords

Sentul Chicken; FSHR; egg production; egg quality; SNP

Abstract

The study was conducted to compare differences in the characteristics of egg production and quality, as well as the identification of FSHR gene polymorphisms in various sentul chickens. The research was conducted by experimental methods using a completely randomized design. The research material used 100 female Sentul chickens aged 16 weeks. The treatment tested was a fixed factor of various Sentul chickens consisting of: “Abu” Sentul, “Emas” Sentul, “Geni” Sentul, “Debu” Sentul, “Batu” Sentul. The variables measured include hen day production, egg weight, and egg quality. The data obtained were analyzed of variance. The primary design used Primer3 based on a database from GeneBank (NM_205079.1), Gallus gallus follicle stimulating hormone receptor (FSHR). The primary base sequence of the FSHR gene was forward/sequence: 5’-CGTGATTCACACACCCTGAC-3 ‘and reverse/sequence: 5’-CCACCTGGACACTTTCCTGT-3’. The DNA sequences were aligned by using BioEdit version 7.7 for identification of the single nucleotide polymorphism (SNP). The analysis of variance showed that various Sentul chickens had insignificant different egg production and quality characteristics (P>0.05). The sequencing results at base length 64 bp showed a mutation from guanine to adenine, and at base 66 bp showed a mutation from cytosine to adenine, so that there were genotypes AA, AC, GA, and GC. The heterozygosity value based on the FSHR gene was 0.594. The results showed that the high heterozygosity of the FSHR Gallus gallus had no effect on the diversity of egg production and quality of various Sentul chickens.

Copyright

© 2022 The Authors. Published by Atlantis Press International B.V.

Open Access

This is an open access article under the CC BY-NC license.

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TY  - CONF
AU  - Ismoyowati Ismoyowati
AU  - DM Saleh
AU  - I Suswoyo
PY  - 2022
DA  - 2022/03/29
TI  - Egg Production Characteristic and the Study of Follicle-stimulating Hormone Receptor Gene on Various of Sentul Chicken
BT  - Proceedings of the International Conference on Improving Tropical Animal Production for Food Security (ITAPS 2021)
PB  - Atlantis Press
SP  - 4
EP  - 9
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220309.002
DO  - 10.2991/absr.k.220309.002
ID  - Ismoyowati2022
ER  -