Proceedings of the International Conference on Improving Tropical Animal Production for Food Security (ITAPS 2021)

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Morphology and Morphometric of Frozen-thawed Pasundan Cattle Sperm Incubated in Extreme pH Media

Authors
Rini Widyastuti1, 2, Diah Nugrahani Pristihadi1, Noer Muhammad Dliyaul Haq1, Sigit Prastowo3, 4, Hera Maheshwari1, Cece Sumantri5, Arief Boediono1, *
1Department of Anatomy, Physiology, and Pharmacology, Faculty of Veterinary Medicine, IPB University, Bogor, Indonesia
2Laboratory of Animal Reproduction and Artificial Insemination, Department of Animal Production, Animal Husbandry Faculty, Universitas Padjadjaran. Jl. Raya Bandung- Sumedang Km. 21, West Java, Indonesia
3Department of Animal Science, Faculty of Agriculture, Universitas Sebelas Maret, Surakarta, Indonesia
4Animal Breeding, Reproduction, and Biostatistics Research Group (ABRE-RG), Universitas Sebelas Maret
5Departmentof Animal Production, Faculty of Animals Science, IPB University, Bogor, Indonesia
*Corresponding author. Email: ab@apps.ipb.ac.id
Corresponding Author
Arief Boediono
Available Online 29 March 2022.
DOI
10.2991/absr.k.220309.010How to use a DOI?
Keywords
sperm; acid; alkaline; morphology; morphometry; Pasundan Cattle
Abstract

An evaluation of sperm morphology and morphometry is useful to predict sperm quality and in the diagnosis of fertile animals. The purpose of this study is to assess the morphology and morphometry of frozen-thawed Pasundan Cattle sperm, as well as the correlation between the two parameters. The sample was obtained from frozen-thawed Pasundan Cattle sperm incubated in pH 4 as acidic media, pH 7.2-7.4 as a control, and pH 11 as alkali media. After 5 minutes of incubation in media, all samples were returned to pH 7.2-7.4 by adding buffer media and immediately evaluated. The results revealed that the percentage of sperm with normal morphology was significantly lower (p<0.05) in the alkali or acidic groups compared to the control. Furthermore, the width of all sperm areas was significantly different in acidic compared to the control and alkali groups (p<0.05). There was no significant difference in treatments or control groups for the entire head and neck area morphometric (p<0.05). Moreover, the width of the sperm tail area displayed the same pattern as all widths of the sperm area. Furthermore, it can be concluded that extreme acidic and alkali media significantly impact on morphometry and morphology of frozen-thawed Pasundan Cattle sperm.

Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article under the CC BY-NC license.

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Volume Title
Proceedings of the International Conference on Improving Tropical Animal Production for Food Security (ITAPS 2021)
Series
Advances in Biological Sciences Research
Publication Date
29 March 2022
ISBN
978-94-6239-557-2
ISSN
2468-5747
DOI
10.2991/absr.k.220309.010How to use a DOI?
Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article under the CC BY-NC license.

Cite this article

risenwbib
TY  - CONF
AU  - Rini Widyastuti
AU  - Diah Nugrahani Pristihadi
AU  - Noer Muhammad Dliyaul Haq
AU  - Sigit Prastowo
AU  - Hera Maheshwari
AU  - Cece Sumantri
AU  - Arief Boediono
PY  - 2022
DA  - 2022/03/29
TI  - Morphology and Morphometric of Frozen-thawed Pasundan Cattle Sperm Incubated in Extreme pH Media
BT  - Proceedings of the International Conference on Improving Tropical Animal Production for Food Security (ITAPS 2021)
PB  - Atlantis Press
SP  - 46
EP  - 50
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220309.010
DO  - 10.2991/absr.k.220309.010
ID  - Widyastuti2022
ER  -