Optimization of Single Cell Protein (SCP) Production using Saccharomyces Cerevisiae by Response Surface Methodology (RSM) on Cell Biomass and Protein Percentage

DOI

10.2991/absr.k.220309.093How to use a DOI?

Keywords

Cell biomass; crude protein; optimization; response surface methodology and single-cell protein

Abstract

This study aims to determine the effect of using molasses, KH₂PO₄ and MgSO₄ media with different concentrations and formulations on S. cerevisiae’s growth in manufacturing single-cell protein (SCP). The method used was the response surface methodology (RSM), consisting of 14 treatment formulations and 2 replications. The test parameters carried out in this study were cell biomass and crude protein content. Obtaining data were analyzed using the expert design program 11 with the response surface methodology (RSM) method and the box-Behnken experimental design. The relationship between fixed variables and independent variables of cell biomass is modeled Y:+6.54-28.63X₁-0.0050X₂-.0112X₃0.4025X₁X₂+0.0900X₁X₃-0.0775X₂X₃, while crude protein is modeled Y:+6.54-28.63X₁-0.0050X₂-0.0112X₃0.4025X₁X₂+0.0900X₁X₃-0.0775X₂X₃. The results showed that the higher the concentration of molasses, the increased cell biomass. The highest cell biomass value was obtained in the addition of 20% molasses to produce 2.33 grams, while the addition of 20% molasses and 0.7% KH₂PO₄ crude protein produced a low crude protein of 6.00%.

Copyright

© 2022 The Authors. Published by Atlantis Press International B.V.

Open Access

This is an open access article under the CC BY-NC license.

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TY  - CONF
AU  - Anis Usfah Prastujati
AU  - Mustofa Hilmi
AU  - Asmaul Khusna
AU  - Osfar Sjofjan
AU  - Muhammad Halim Natsir
AU  - Shabrina Ulfa Talitha
PY  - 2022
DA  - 2022/03/29
TI  - Optimization of Single Cell Protein (SCP) Production using Saccharomyces Cerevisiae by Response Surface Methodology (RSM) on Cell Biomass and Protein Percentage
BT  - Proceedings of the International Conference on Improving Tropical Animal Production for Food Security (ITAPS 2021)
PB  - Atlantis Press
SP  - 483
EP  - 489
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220309.093
DO  - 10.2991/absr.k.220309.093
ID  - Prastujati2022
ER  -