Proceedings of the International Conference on Improving Tropical Animal Production for Food Security (ITAPS 2021)

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Optimization of Single Cell Protein (SCP) Production using Saccharomyces Cerevisiae by Response Surface Methodology (RSM) on Cell Biomass and Protein Percentage

Authors
Anis Usfah Prastujati1, *, Mustofa Hilmi1, Asmaul Khusna1, Osfar Sjofjan2, Muhammad Halim Natsir2, Shabrina Ulfa Talitha1
1Politeknik Negeri Banyuwangi
2Universitas Brawijaya
*Corresponding author. Email: anis.usfah@poliwangi.ac.id
Corresponding Author
Anis Usfah Prastujati
Available Online 29 March 2022.
DOI
10.2991/absr.k.220309.093How to use a DOI?
Keywords
Cell biomass; crude protein; optimization; response surface methodology and single-cell protein
Abstract

This study aims to determine the effect of using molasses, KH2PO4 and MgSO4 media with different concentrations and formulations on S. cerevisiae’s growth in manufacturing single-cell protein (SCP). The method used was the response surface methodology (RSM), consisting of 14 treatment formulations and 2 replications. The test parameters carried out in this study were cell biomass and crude protein content. Obtaining data were analyzed using the expert design program 11 with the response surface methodology (RSM) method and the box-Behnken experimental design. The relationship between fixed variables and independent variables of cell biomass is modeled Y:+6.54-28.63X1-0.0050X2-.0112X30.4025X1X2+0.0900X1X3-0.0775X2X3, while crude protein is modeled Y:+6.54-28.63X1-0.0050X2-0.0112X30.4025X1X2+0.0900X1X3-0.0775X2X3. The results showed that the higher the concentration of molasses, the increased cell biomass. The highest cell biomass value was obtained in the addition of 20% molasses to produce 2.33 grams, while the addition of 20% molasses and 0.7% KH2PO4 crude protein produced a low crude protein of 6.00%.

Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article under the CC BY-NC license.

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Volume Title
Proceedings of the International Conference on Improving Tropical Animal Production for Food Security (ITAPS 2021)
Series
Advances in Biological Sciences Research
Publication Date
29 March 2022
ISBN
10.2991/absr.k.220309.093
ISSN
2468-5747
DOI
10.2991/absr.k.220309.093How to use a DOI?
Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article under the CC BY-NC license.

Cite this article

risenwbib
TY  - CONF
AU  - Anis Usfah Prastujati
AU  - Mustofa Hilmi
AU  - Asmaul Khusna
AU  - Osfar Sjofjan
AU  - Muhammad Halim Natsir
AU  - Shabrina Ulfa Talitha
PY  - 2022
DA  - 2022/03/29
TI  - Optimization of Single Cell Protein (SCP) Production using Saccharomyces Cerevisiae by Response Surface Methodology (RSM) on Cell Biomass and Protein Percentage
BT  - Proceedings of the International Conference on Improving Tropical Animal Production for Food Security (ITAPS 2021)
PB  - Atlantis Press
SP  - 483
EP  - 489
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220309.093
DO  - 10.2991/absr.k.220309.093
ID  - Prastujati2022
ER  -