Proceedings of the International Conference on Improving Tropical Animal Production for Food Security (ITAPS 2021)

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The Longevity of Sexed Sperm after Sexing with Several Combination of Bovine Serum Albumin (BSA) Concentration

Authors
Nurcholidah Solihati1, *, Siti Darodjah Rasad1, Nena Hilmia1
1Department of Animal Production, Faculty of Animal Husbandry, Universitas Padjadjaran*Corresponding author.
*Corresponding author. Email: nurcholidah@yahoo.com
Corresponding Author
Nurcholidah Solihati
Available Online 29 March 2022.
DOI
10.2991/absr.k.220309.029How to use a DOI?
Keywords
Longevity; Sexed sperm; Albumin
Abstract

The aim of the research was to evaluate the longevity of sexed sperm of local ram after sexing with different combination of Bovine Serum Albumin (BSA) concentration in chilled and post thawed sexed sperm. The object of the research was 10 ejaculated semen from 3 years old local ram. The method was a Completely Randomize Design (CRD) with four treatments combination of BSA concentration on upper layer and bottom layer (T1: 3% and 6%, T2: 4% and 8%, T3: 5% and 10%, dan T4: 6% and 12%), each treatment was repeated 10 times. Result showed that there is significant difference (P<0.05) in sperm longevity both in chilled and post thawed sexed sperm. Sperm longevity in chilled sexed sperm on of upper layer in T1, T2, T3 and T4 were 229.60±16.13, 231.90±10.27, 265.40±15.01, and 196.60±19.21 hours, respectively, as well as on bottom layer in T1, T2, T3 and T4 were 207.70±31.18, 207.90±31.47, 239.10±30.82 and 181.60±31.81 hours, respectively. Sperm longevity in post thawed sexed sperm on upper layer in T1, T2, T3 and T4 were 142.80±2.68, 142.20±1.64, 147.00±3.00, and 142.20±1.64 hours, respectively, and then on bottom layer were 121.80±2.68, 120.60±3.29, 125.40±3.29 and 118.80±1.64 hours, respectively. In conclusion, the combination of BSA concentration of 5% and 10% at upper and bottom layer resulting longest sperm longevity both in chilled and post thawed sexed sperm.

Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article under the CC BY-NC license.

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Volume Title
Proceedings of the International Conference on Improving Tropical Animal Production for Food Security (ITAPS 2021)
Series
Advances in Biological Sciences Research
Publication Date
29 March 2022
ISBN
10.2991/absr.k.220309.029
ISSN
2468-5747
DOI
10.2991/absr.k.220309.029How to use a DOI?
Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article under the CC BY-NC license.

Cite this article

risenwbib
TY  - CONF
AU  - Nurcholidah Solihati
AU  - Siti Darodjah Rasad
AU  - Nena Hilmia
PY  - 2022
DA  - 2022/03/29
TI  - The Longevity of Sexed Sperm after Sexing with Several Combination of Bovine Serum Albumin (BSA) Concentration
BT  - Proceedings of the International Conference on Improving Tropical Animal Production for Food Security (ITAPS 2021)
PB  - Atlantis Press
SP  - 141
EP  - 144
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220309.029
DO  - 10.2991/absr.k.220309.029
ID  - Solihati2022
ER  -