Parasitological and Molecular Detection of Babesiosis in Cattle and Buffalo in West and Central Java

DOI

10.2991/absr.k.220207.028How to use a DOI?

Keywords

Babesia bovis; Babesia bigemina; single PCR; duplex PCR; Thin blood smear

Abstract

Babesia sp are the intra-erythrocytic protozoan parasite causes Bovine babesiosis. Babesia bovis and Babesia bigemina are two species that commonly infest cattle. A conventional parasitological technique is commonly used to diagnose Babesia sp. This technique, however, has limitations in subclinical infections. Polymerase chain reaction (PCR) has a high sensitivity and can identify parasites at much lower concentrations. The goal of this study was to detect B. bovis and B. bigemina in blood cattle and buffaloes from fields using the conventional Giemza Staining Thin Blood Smear method (GSTBS). In addition, the study compared the effectiveness of a single and duplex PCR in detecting parasites. One hundred and ninety-eight blood samples from cattle and buffalo were collected in Bogor (West Java), Pemalang, and Brebes (Central Java). The parasite’s existence was determined using a thin blood smear. Furthermore, the PCR assay was employed with primers Bovar 2A and Bg3/4 specific for B. bovis and B. bigemina, yielding amplified products of 166 and 689 bp, respectively. Based on parasitological findings, 14 of 198 blood samples (7.1%) tested positive for Babesia sp. The results of a single PCR demonstrated that 21,21% (42/198) of the samples were positive for B. bovis, whereas duplex PCR successfully identified B. bovis and B. bigemina in 72 samples (36,36%). This finding indicated that duplex PCR is more efficient and cost-effective than GSTBS and single PCR for surveying babesiosis in epidemiological studies.

Copyright

© 2022 The Authors. Published by Atlantis Press International B.V.

Open Access

This is an open access article under the CC BY-NC license.

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TY  - CONF
AU  - D H Sawitri
AU  - A H Wardhana
AU  - F Ekawasti
AU  - D A Dewi
PY  - 2022
DA  - 2022/02/24
TI  - Parasitological and Molecular Detection of Babesiosis in Cattle and Buffalo in West and Central Java
BT  - Proceedings of the 9th International Seminar on Tropical Animal Production (ISTAP 2021)
PB  - Atlantis Press
SP  - 135
EP  - 140
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220207.028
DO  - 10.2991/absr.k.220207.028
ID  - Sawitri2022
ER  -