Proceedings of the 4th International Seminar on Science and Technology (ISST 2022)

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Isolation and Characterization of Endophytic Actinomycetes on Onion Root (Allium Ascalonicum L.) Palu Cultivar

Authors
Ayu Lestari1, *, Abd. Rahmat Sapa1, Meryany Ananda1, I. Nengah Suwastika1
1Department of Biology Faculty of Sciences, Tadulako University, Jl.Soekarno Hatta KM 9, Palu, 94118, Indonesia
*Corresponding author. Email: ayulestari4234@gmail.com
Corresponding Author
Ayu Lestari
Available Online 22 August 2023.
DOI
10.2991/978-94-6463-228-6_26How to use a DOI?
Keywords
Actinomycetes; Onion; growth rate; growth medium
Abstract

Endophytic microbes are microbes that during their entire life cycle are in plant tissues. Actinomycetes is one of the endophytic microbes that produce secondary metabolites and can be found in the roots of onions. This study aimed to isolate, characterize and determine the growth rate of endophytic actinomycetes from Allium ascalonicum L. Palu cultivar. The isolation was done by spread plate method using SCA (Starch casein agar) medium, then purified and characterized their properties macroscopically (morphology of colony) and microscopically (morphology of cell) before it is determined their growth rate. Actinomycetes were isolated from the roots of onion namely, JO1, JO2, and JO3 which showed different in characters of each isolate. The result also showed that Actinomycetes growth rate was quicker on onion root extract than it was in SCB (Starch casein broth) medium. Based on that data, it can be concluded that endophytic Actinomycetes can be grown in onion root extract medium.

Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the 4th International Seminar on Science and Technology (ISST 2022)
Series
Advances in Physics Research
Publication Date
22 August 2023
ISBN
978-94-6463-228-6
ISSN
2352-541X
DOI
10.2991/978-94-6463-228-6_26How to use a DOI?
Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

risenwbib
TY  - CONF
AU  - Ayu Lestari
AU  - Abd. Rahmat Sapa
AU  - Meryany Ananda
AU  - I. Nengah Suwastika
PY  - 2023
DA  - 2023/08/22
TI  - Isolation and Characterization of Endophytic Actinomycetes on Onion Root (Allium Ascalonicum L.) Palu Cultivar
BT  - Proceedings of the 4th International Seminar on Science and Technology (ISST 2022)
PB  - Atlantis Press
SP  - 228
EP  - 234
SN  - 2352-541X
UR  - https://doi.org/10.2991/978-94-6463-228-6_26
DO  - 10.2991/978-94-6463-228-6_26
ID  - Lestari2023
ER  -