Proceedings of the International Seminar on Promoting Local Resources for Sustainable Agriculture and Development (ISPLRSAD 2020)

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Combined Effect of Methanol Extract of Persea americana Seeds and Cassia alata Leaves Against Staphylococcus epidermidis

Authors
Morina Adfa, Ni Made Candra Gusatyana, Andre Mudyanto, Avidlyandi Avidlyandi, Dwita Oktiarni, S. Salprima Yudha
Corresponding Author
Morina Adfa
Available Online 11 June 2021.
DOI
10.2991/absr.k.210609.006How to use a DOI?
Keywords
Antibacterial, combination, Cassia alata, Persea americana, Staphylococcus epidermidis
Abstract

The aim of this study was to analyze the individual and combined antimicrobial effects of avocado seed (Persea americana) and ketepeng cina leaf (Cassia alata) methanol extracts against Staphylococcus epidermidis using the Kirby Bauer assay. Variations in the proportions of P. americana to C. alata (A: K) extracts were 4: 0 mg/mL, 8: 0 mg/mL, 12: 0 mg/mL, 0: 4 mg/mL, 0: 8 mg/mL, 0: 12 mg/mL, 4: 4 mg/mL, 4: 8 mg/mL, 4: 12 mg/mL, 8: 4 mg/mL, and 12: 4 mg/mL respectively, and the tests were executed in four replicates. The positive controls used were Clindamycin and Chloramphenicol, while the negative was DMSO. After 24 hours of incubation, zones of inhibition were exhibited by the experimental group and positive control, but not by the negative test. The largest diameters from the methanol extract of P. americana seeds and C. alata leaves singly at strengths of 12 mg/mL were 5.96 mm and 9 mm, while the mixture at various concentrations showed the pattern of growth organism as an indifferent (additive) effect.

Copyright
© 2021, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the International Seminar on Promoting Local Resources for Sustainable Agriculture and Development (ISPLRSAD 2020)
Series
Advances in Biological Sciences Research
Publication Date
11 June 2021
ISBN
10.2991/absr.k.210609.006
ISSN
2468-5747
DOI
10.2991/absr.k.210609.006How to use a DOI?
Copyright
© 2021, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

risenwbib
TY  - CONF
AU  - Morina Adfa
AU  - Ni Made Candra Gusatyana
AU  - Andre Mudyanto
AU  - Avidlyandi Avidlyandi
AU  - Dwita Oktiarni
AU  - S. Salprima Yudha
PY  - 2021
DA  - 2021/06/11
TI  - Combined Effect of Methanol Extract of Persea americana Seeds and Cassia alata Leaves Against Staphylococcus epidermidis
BT  - Proceedings of the International Seminar on Promoting Local Resources for Sustainable Agriculture and Development (ISPLRSAD 2020)
PB  - Atlantis Press
SP  - 36
EP  - 40
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.210609.006
DO  - 10.2991/absr.k.210609.006
ID  - Adfa2021
ER  -