Selection of Culture Medium and Incubation Time for Growth and Production of Beauvericin by Local Beauveria bassiana

DOI

10.2991/absr.k.210609.011How to use a DOI?

Keywords

Beauveria bassiana, beauvericin, culture medium, incubation time, beauvericin specific production

Abstract

Beauveria bassiana, an entomopathogenic fungus, is a high producer of beauvericin (BEA). BEA is a proven and useful compound as a mycoinsecticide for plant pest control and a potential antifungal and anticancer agent for human. BEA produced by Beauveria bassiana fungi, mainly found as an intracellular product, means its production is dependent on the growth of the fungi in the culture medium. This study investigated four culture mediums and two incubation times to enhance growth and BEA production by Beauveria bassiana isolated from the infected insects in Kediri and Mojokerto, East Java, Indonesia. The four culture mediums were Potato Dextrose Broth (PDB), Yeast and Malt Extract Broth (YMB), Malt Extract Broth (MB), and Fusarium Defined Medium (FDM), and two incubation times were 6 and 12 days. The biomass and BEA production were studied in a batch culture without agitation. Our data shows that YMB was the optimum culture medium to produce high biomass of the fungal mycelium in both strains for 6 and 12 days of incubation. However, instead of in YMB, the highest BEA production for both strains was obtained from Beauveria bassiana grown in PDB for 6 days and in MB for 12 days. Correlation between biomass and BEA production in every culture medium is then calculated to see the BEA specific production. The highest BEA specific production has resulted from Beauveria bassiana grown for 12 days in MB medium with BEA yield was 103,42 mg/L and 237,49 mg/L for strain Kediri and Mojokerto, respectively.

Copyright

© 2021, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Y. P. Roswanjaya
AU  - N. A. Saryanah
AU  - W. Nawfetrias
AU  - H. Rosdayanti
AU  - A. L. Putri
PY  - 2021
DA  - 2021/06/11
TI  - Selection of Culture Medium and Incubation Time for Growth and Production of Beauvericin by Local Beauveria bassiana
BT  - Proceedings of the International Seminar on Promoting Local Resources for Sustainable Agriculture and Development (ISPLRSAD 2020)
PB  - Atlantis Press
SP  - 59
EP  - 65
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.210609.011
DO  - 10.2991/absr.k.210609.011
ID  - Roswanjaya2021
ER  -