Lipid Production from Zygosaccharomyces siamensis AP1 Using Glycerol as a Carbon Source

DOI

10.2991/absr.k.210810.014How to use a DOI?

Keywords

C/N ratio, cheap carbon source, oleaginous yeast, production profile, wild honey

Abstract

Yeasts are considered as potential lipid producer because they are easy to cultivate, able to grow in high cell densities and can produce high concentrations of lipids. Previously we had isolated a promising lipid producing yeast, Zygosaccharomyces siamensis AP1, from wild honey of Central Sulawesi, Indonesia. The strain was able to produce around 19% (w/w biomass) of lipid on glucose. However, its ability to produce lipid on other types of carbon source is still not studied. In this study, we compared the strain ability to produce lipid on glycerol and glucose using different C/N ratio. We also investigated optimum temperature, pH, and growth time for lipid production on glycerol. We found that growing the strain on glycerol with C/N ratio 120 gave the highest lipid content (29.7%). We also found that optimum growth temperature and pH are 30°C and 5, respectively. Maximum lipid biomass, 0.28 g/L, was produced when the strain was grown in the optimum conditions for 72 hours. In conclusion, our results suggest that glycerol is a potential cheap carbon source for lipid production from Z. siamensis AP1. We suggest further study to increase lipid yield either by fermentation or strain improvement.

Copyright

© 2021, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Download article (PDF)

TY  - CONF
AU  - M. Ilmi
AU  - M. Siswontoro
PY  - 2021
DA  - 2021/08/12
TI  - Lipid Production from Zygosaccharomyces siamensis AP1 Using Glycerol as a Carbon Source
BT  - Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)
PB  - Atlantis Press
SP  - 71
EP  - 75
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.210810.014
DO  - 10.2991/absr.k.210810.014
ID  - Ilmi2021
ER  -