Development of Methods For Quantitative Determination of Polyphenols in Grass Pentaphylloides Fruticosa (L.) O. Schwarz.

DOI

10.2991/isils-19.2019.88How to use a DOI?

Keywords

P. fruticosa L. herb, RP HPLC, quercetin glycosides, kaempferol glycosides, rhamnetin glycosides, proacyanidins, elagic acid, ellagotannins

Abstract

The purpose of this study was to develop a method of quali-tative and quantitative assessment of biologically active sub-stances of P. fruticosa L. herb. As object of study was used P. fruticosa L. herb. We used the method of high performance liquid chromatography in reversed-phase variant. As a result of chromatography, it was found that the chromatographic system used can effectively separate and identify the poly-phenol complex of the herb P. fruticosus L. The chromato-gram shows that the polyphenol complex of the herb P. fruti-cosus L. consists of the glycosides quercetin, kaempferol, rhamnetin, procyanidins, ellagotannins and ellagic acid and chlorogenic acid. Aglycones were found in the hydrolysis products: quercetin, kempferol, ellagic acid, and trace amounts of rhamnetin. Thus, as a result of this study, the composition of the polyphenolic complex of the herb P. fruti-cosus L. was established, including the glycosides of querce-tin, kaempferol, rhamnetin, proacyanidins, ellagic acid and ellagotannins. A method has been developed for the quanti-tative determination of the sum of aglycones of grass poly-phenols of P. fruticosus L., which is based on the principle of extraction with the simultaneous hydrolysis of glycosides of polyphenols to aglycones and their subsequent chromato-graphic determination by high performance liquid chroma-tography and quantification using absolute calibration.

Copyright

© 2019, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Elena Zhilyakova
AU  - Oleg Novikov
AU  - Dmitriy Pisarev
AU  - Liliya Zolotareva
PY  - 2019/11
DA  - 2019/11
TI  - Development of Methods For Quantitative Determination of Polyphenols in Grass Pentaphylloides Fruticosa (L.) O. Schwarz.
BT  - Proceedings of the 1st International Symposium Innovations in Life Sciences (ISILS 2019)
PB  - Atlantis Press
SP  - 89
EP  - 93
SN  - 2468-5747
UR  - https://doi.org/10.2991/isils-19.2019.88
DO  - 10.2991/isils-19.2019.88
ID  - Zhilyakova2019/11
ER  -