Peculiarities of Use of Bentonite Clay at Solid-Phase Purification of Anthocyanins and Flavonoids from Leaves of Plants

DOI

10.2991/isils-19.2019.21How to use a DOI?

Keywords

anthocyanins, Cercis canadensis L., leaves, SPE, flavonoids, clay

Abstract

Anthocyanins of red colored leaves of Cercis canadensis L. were found to be 3-glucosides of five common anthocyanidins - of delphinidin, cyaniding, petunidin, peonidin and malvidin with overall accumulation more than 350 mg per 100 g of FW in early summer. The content of anthocyanins gradually drops to total disappearance at the late September. Extract of anthocyanins may content a large amount of flavonoids that are not separated during solid phase extraction (SPE) on C18-silica syringe cartridges. In the paper, the ability of bentonite clay as a sorbent for SPE of anthocyanins was investigated. Non-charged flavonoids were shown to compete with anthocyanins during sorption. Meanwhile sorption ability of anthocyanins markedly exceeds that of non-charged flavonoids. The method for anthocyanins and non-charged flavonoids is proposed.

Copyright

© 2019, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Andrey Doronin
AU  - Victor Deineka
AU  - Ludmila Deineka
AU  - Mikhail Tretiakov
AU  - Valery Tokhtar
AU  - Andrey Chulkov
PY  - 2019/11
DA  - 2019/11
TI  - Peculiarities of Use of Bentonite Clay at Solid-Phase Purification of Anthocyanins and Flavonoids from Leaves of Plants
BT  - Proceedings of the 1st International Symposium Innovations in Life Sciences (ISILS 2019)
PB  - Atlantis Press
SP  - 192
EP  - 195
SN  - 2468-5747
UR  - https://doi.org/10.2991/isils-19.2019.21
DO  - 10.2991/isils-19.2019.21
ID  - Doronin2019/11
ER  -