Addition of Fish Oil in the Formulation of Alginate Granules as an Alternative for Oral Fish Vaccines

Desy Sugiani; Taukhid Taukhid; Angela Mariana Lusiastuti; Yani Aryati; Uni Purwaningsih; Hessy Novita; Tuti Sumiati; Nunak Nafiqoh; Lila Gardenia; Septyan Andriyanto

doi:10.2991/978-94-6463-942-1_8

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Addition of Fish Oil in the Formulation of Alginate Granules as an Alternative for Oral Fish Vaccines

Authors

Desy Sugiani¹^{, *}, Taukhid Taukhid², Angela Mariana Lusiastuti¹, Yani Aryati³, Uni Purwaningsih¹, Hessy Novita¹, Tuti Sumiati¹, Nunak Nafiqoh⁴, Lila Gardenia⁴, Septyan Andriyanto⁵

¹Research Center for Veterinary Science, Research Organization for Health, National Research and Innovation Agency, Jakarta, Indonesia

²Research Center for Fisheries, Research Organization for Earth and Maritime, National Research and Innovation Agency, Jakarta, Indonesia

³Research Center for Applied Microbiology, Research Organization for Life Sciences and Environment, National Research and Innovation Agency, Jakarta, Indonesia

⁴Directorate of Scientific and Collection Management, National Research and Innovation Agency, Jakarta, Indonesia

⁵Organization and Human Resources Bureau, National Research and Innovation Agency, Jakarta, Indonesia

^*Corresponding author. Email: desysugiani@gmail.com

Corresponding Author

Desy Sugiani

Available Online 29 December 2025.

DOI: 10.2991/978-94-6463-942-1_8 How to use a DOI?
Keywords: Alginate; Aeromonas hydrophila; Delivery system; Granules; Oral Fish Vaccines
Abstract: Alginate is widely used for encapsulation material in the development of fish drugs enrichment. In this study, alginate was developed to construct granules containing fish vaccines. The preparation of granules using alginate with food and pharmaceutical grade was compared to obtain the best shape. The addition of fish oil was also carried out with different concentrations of 10 mL.L⁻¹, 5 mL.L⁻¹, and 1 mL.L⁻¹ to obtain optimal physical properties and acted as an effective attractant. The results showed that pharmaceutical grade alginate and the addition of 10 mL.L⁻¹ fish oil were the best in forming granules. Based on the bacterial contamination test of Esherichia coli and Salmonella sp. were negative. The feeding trial to Clarias gariepinus showed that the granules reached the anterior intestine after 1 hours post feeding and 3 hours post intake the granules were found in posterior intestine and digested. The granules were safely digested in the intestinal tract of Clarias gariepinus. While this formulation shows promise as a vaccine delivery vehicle, its efficacy in inducing a protective immune response requires further investigation.
Open Access: Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title: Proceedings of the 3rd International Seminar on Fish and Fisheries Sciences (ISFFS 2025)
Series: Advances in Biological Sciences Research
Publication Date: 29 December 2025
ISBN: 978-94-6463-942-1
ISSN: 2468-5747
DOI: 10.2991/978-94-6463-942-1_8 How to use a DOI?
Open Access: Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

ris enw bib

TY  - CONF
AU  - Desy Sugiani
AU  - Taukhid Taukhid
AU  - Angela Mariana Lusiastuti
AU  - Yani Aryati
AU  - Uni Purwaningsih
AU  - Hessy Novita
AU  - Tuti Sumiati
AU  - Nunak Nafiqoh
AU  - Lila Gardenia
AU  - Septyan Andriyanto
PY  - 2025
DA  - 2025/12/29
TI  - Addition of Fish Oil in the Formulation of Alginate Granules as an Alternative for Oral Fish Vaccines
BT  - Proceedings of the 3rd International Seminar on Fish and Fisheries Sciences (ISFFS 2025)
PB  - Atlantis Press
SP  - 72
EP  - 80
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-942-1_8
DO  - 10.2991/978-94-6463-942-1_8
ID  - Sugiani2025
ER  -

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