Proceedings of the 6th International Seminar of Animal Nutrition and Feed Science (ISANFS 2021)

Crude Aflatoxin B1 Production Using Maize and Rice Substrates for Animal Research

Authors
Muhsin Al Anas1, Lies Mira Yusiati1, Cuk Tri Noviandi1, Ali Agus1, *
1Department of Animal Nutrition and Feed Science, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, Indonesia 55821
*Corresponding author. Email: aliagus@ugm.ac.id
Corresponding Author
Ali Agus
Available Online 11 April 2022.
DOI
10.2991/absr.k.220401.004How to use a DOI?
Keywords
Aflatoxin B1; Production; Substrat; Maize dan Rice
Abstract

This study aimed to develop a simple method of crude aflatoxin (AF) B1 production for animal research. A. flavus was planted on potato dextrose agar (PDA) medium as an isolate to be grown on maize and rice substrates at a concentration of 107/mL. Maize was added to water until the water content reached 25%, while rice was soaked in water at a ratio of 1 mL of water for 2 g of rice for 1 hour. The rice and maize were sterilized using an autoclave. A total of 250 g of each substrate was inoculated with A. flavus FNCC 6122. Incubation was carried out for 6 days at 30°C at a humidity of 80-90%. On the second day of the incubation period, 1 mL of water was added to the substrates. At the end of the incubation period, crude AF samples were analyzed by enzyme-linked immunosorbent assay (ELISA). The results showed that level of AFB1 on rice higher than maize (P<0.001). The AFB1 production was 3229 µg/kg in rice and 1153 µg/kg in maize. Rice and maize can be used as a substrate source for crude AFB1 production for animal research.

Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license.

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Volume Title
Proceedings of the 6th International Seminar of Animal Nutrition and Feed Science (ISANFS 2021)
Series
Advances in Biological Sciences Research
Publication Date
11 April 2022
ISBN
978-94-6239-562-6
ISSN
2468-5747
DOI
10.2991/absr.k.220401.004How to use a DOI?
Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license.

Cite this article

TY  - CONF
AU  - Muhsin Al Anas
AU  - Lies Mira Yusiati
AU  - Cuk Tri Noviandi
AU  - Ali Agus
PY  - 2022
DA  - 2022/04/11
TI  - Crude Aflatoxin B₁ Production Using Maize and Rice Substrates for Animal Research
BT  - Proceedings of the 6th International Seminar of Animal Nutrition and Feed Science (ISANFS 2021)
PB  - Atlantis Press
SP  - 17
EP  - 21
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220401.004
DO  - 10.2991/absr.k.220401.004
ID  - AlAnas2022
ER  -