Proceedings of the 2nd International Interdisciplinary Conference on Environmental Sciences and Sustainable Developments 2022 Environment and Sustainable Development (IICESSD-ESD 2022)

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Strategy Propagation of Coffea arabica L. by Tissue Culture Techniques

Authors
Rizka Amaliah R. Maruka1, *, Meryany Ananda1, Eny Yuniati1, Asri Pirade Paserang1
1Depertement of Biology Faculty of Math and Science, Tadulako University, Palu, Central Sulawesi, 94418, Indonesia
*Corresponding author. Email: rizkaamaliahmaruka@gmail.com
Corresponding Author
Rizka Amaliah R. Maruka
Available Online 24 January 2024.
DOI
10.2991/978-94-6463-334-4_35How to use a DOI?
Keywords
2.4 D; BAP; NAA; Callus; Coffea
Abstract

The research of in vitro callus induction on arabica coffee (Coffea arabica L.) with 2.4 D, BAP, and NAA, was conducted in Biotechnology and Genetics Laboratory, Tissue Culture Unit, Department of Biology, Faculty of Mathematics and Natural Sciences. Tadulako University. The purpose of this research was determine a callus induction method for Coffea arabica L. in vitro propagation using a variety of mediums. A completely randomized Design (CRD) with 6 treatments and 3 replications was used in this experimental. BAP 1 ppm + NAA 0.2 ppm, BAP 1.5 ppm + NAA 0.2 ppm, BAP 2 ppm + NAA 0.2 ppm, 2.4 D 1 ppm + BAP 0.2 ppm, 2, 4 D 1.5 ppm + BAP 0.2 ppm, and 2.4 D 2 ppm + BAP were the different treatments. 0.2 ppm in. Based on the results, it can be determined that 2.4D 2 ppm + 0. 2 ppm BAP with a 100% callus percentage was the proper and effective of treatment. With a crumb texture and a white callus appearance.

Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the 2nd International Interdisciplinary Conference on Environmental Sciences and Sustainable Developments 2022 Environment and Sustainable Development (IICESSD-ESD 2022)
Series
Advances in Biological Sciences Research
Publication Date
24 January 2024
ISBN
10.2991/978-94-6463-334-4_35
ISSN
2468-5747
DOI
10.2991/978-94-6463-334-4_35How to use a DOI?
Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

risenwbib
TY  - CONF
AU  - Rizka Amaliah R. Maruka
AU  - Meryany Ananda
AU  - Eny Yuniati
AU  - Asri Pirade Paserang
PY  - 2024
DA  - 2024/01/24
TI  - Strategy Propagation of Coffea arabica L. by Tissue Culture Techniques
BT  - Proceedings of the 2nd International Interdisciplinary Conference on Environmental Sciences and Sustainable Developments 2022 Environment and Sustainable Development (IICESSD-ESD 2022)
PB  - Atlantis Press
SP  - 205
EP  - 208
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-334-4_35
DO  - 10.2991/978-94-6463-334-4_35
ID  - Maruka2024
ER  -