Establishment of a GFP Fluorescence Labeled GLP-1 Receptor Cell Line

DOI

10.2991/iea-15.2015.170How to use a DOI?

Keywords

GLP-1receptor; GLP-1-GFP/U2OS cell line; Type2 diabetics.

Abstract

To establish a green fluorescent protein (GFP)-based cellular model for screening GLP-1 receptor ligands from Traditional Chinese Medicinal herbs. A eukaryotic expression vector pCMV6-GLP-1R-GFP was constructed and transfected into U20S cells. A stable expressing GLP-1R-GFP U20S cell line was established and the expression of GLP-1 receptor was confirmed by Western Blots with a GLP-1 specific antibody. The activities of expressed GLP-1-GFP were investigated with the cellular redistribution of green fluorescence after stimulation with GLP-1 analogs in the absence or presence of a GLP-1 antagonist. The sensitivity and specificity of this cell line was qualified and suitable for GLP-1 receptor ligand screening.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Li Ming-Rui
AU  - Ji Xiu-Ling
AU  - Zhang Min
AU  - Chang Kwen-Jen
AU  - Liu Dong-Bo
AU  - Wei Yun-Lin
PY  - 2015/09
DA  - 2015/09
TI  - Establishment of a GFP Fluorescence Labeled GLP-1 Receptor Cell Line
BT  - Proceedings of the AASRI International Conference on Industrial Electronics and Applications (2015)
PB  - Atlantis Press
SP  - 682
EP  - 685
SN  - 2352-5401
UR  - https://doi.org/10.2991/iea-15.2015.170
DO  - 10.2991/iea-15.2015.170
ID  - Ming-Rui2015/09
ER  -