In Vitro Chitosan Hydrogel Based Metronidazole Cytotoxicity Test on 3t3 Fibroblast Viability

DOI

10.2991/idsm-17.2018.44How to use a DOI?

Keywords

metronidazole, viability, cytotoxicity, fibroblast, chitosan.

Abstract

The objective of this study is to determine the in vitro cytotoxicity effect of chitosan hydrogel-based metronidazole on fibroblast. Chitosan hydrogel-based metronidazole(0.12%, 0.15%, 0.25%) and chitosan without metronidazole are made by dissolving lactic acid to reach a gel consistency, and then adding powdered metronidazole according to the concentration. 3T3 fibroblast cells were cultured in well mikroplate containing RPMI-1640 media inside an incubator. The cell viability tests were carried out using theMTT assay method and repeated five times. The colour degradation was read with a microplate reader. The mean viability of fibroblasts mixed with chitosan hydrogel-based metronidazole at concentration of 0.12%, 0.15%, and 0.25%, and chitosan without metronidazole were 96%, 93%, 91% and 98.8% respectively. ANOVA statistical test results for the 0.12% concentration group, where p < 0.05 represents the cell’s viability, significantly differs from the results for the 0.15% and 0.25% concentration groups. Chitosan hydrogel-based metronidazole shows non toxic effects on fibroblast cells.

Copyright

© 2018, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Hilma Rasni
AU  - Irma Ervina
AU  - Syafruddin Ilyas
PY  - 2018/05
DA  - 2018/05
TI  - In Vitro Chitosan Hydrogel Based Metronidazole Cytotoxicity Test on 3t3 Fibroblast Viability
BT  - Proceedings of the 11th International Dentistry Scientific Meeting (IDSM 2017)
PB  - Atlantis Press
SP  - 330
EP  - 335
SN  - 2468-5739
UR  - https://doi.org/10.2991/idsm-17.2018.44
DO  - 10.2991/idsm-17.2018.44
ID  - Rasni2018/05
ER  -