Virulence of periodontopathogens Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis: micro-computed tomography and inflammatory cytokine analysis

DOI

10.2991/idsm-17.2018.42How to use a DOI?

Keywords

Periodontal disease, crude protein of A. actinomycetemcomitans, P. gingivalis lipopolysaccharides, inflammatory cytokine, μ-CT

Abstract

The objective of this study is to determine the difference between A. actinomy-cetemcomitans and P. gingivalis virulence as the cause of periodontal disease. Thirty male Wistar rats were divided into six groups. Ten rats were induced by 10 ug/mL P. gingivalis lipopolysaccharides (LPS), and the other ten rats were induced by 10 ug/mL crude proteins of A. actinomycetemcomitans. The injection was performed three times for six weeks in the interdental area between the first and the second mandibular right molar by using a 30gauge needle. On the third and seventh days, five rats from each group were decapitated and observed with mi-cro-computed tomography (μ-CT) to determine the severity of alveolar bone re-sorption. The immunohistochemical method was used to find the expression of macrophage inflammatory cytokines (IL-1β and TNF-α). The ANOVA test was performed to determine the difference among each group. The μ-CT image ob-servation of each group revealed that the severity of alveolar bone resorption was different in each group. In the group that was induced by the crude proteins of A. actinomycetemcomitans, resorption was more advanced than those in the LPS and the control group. A significant increase (p<0.05) was found in IL-1β and TNF-α expressions after induction in both groups on the third and seventh days, with the highest cytokine expression observed on the seventh day. The expres-sions of IL-1β and TNF-α in the group that was induced by the crude proteins of A. actinomycetemcomitans were significantly higher than those in the LPS group. The crude proteins of A. actinomycetemcomitans showed a greater level of viru-lence than the P. gingivalis LPS in either alveolar bone resorption or inflammatory cytokine expression.

Copyright

© 2018, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Melok A. Wahyukundari
AU  - Desi S. Sari
AU  - Peni Pujiastuti
AU  - Depi Praharani
AU  - Yuliana M.D Arina
AU  - Tantin Ermawati
AU  - Fourier D.E Latief
PY  - 2018/05
DA  - 2018/05
TI  - Virulence of periodontopathogens Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis: micro-computed tomography and inflammatory cytokine analysis
BT  - Proceedings of the 11th International Dentistry Scientific Meeting (IDSM 2017)
PB  - Atlantis Press
SP  - 312
EP  - 321
SN  - 2468-5739
UR  - https://doi.org/10.2991/idsm-17.2018.42
DO  - 10.2991/idsm-17.2018.42
ID  - Wahyukundari2018/05
ER  -