Identification of Pathogenic Bacteria in Snapper Fish (Lutjanus sp.) from Banda Aceh Waters

DOI

10.2991/absr.k.210420.033How to use a DOI?

Keywords

Aeromonas sp, pathogenic bacteria, snapper fish, 16S rRNA gene

Abstract

Snapper (Lutjanus sp.), which is an important marine fish resource, is known to have relatively low movement activity and form relatively small groups. These characteristics make these fish susceptible to various diseases caused by pathogenic bacteria. This study aims to identify the pathogenic bacterial species that contaminate snapper based on 16S rRNA gene analysis. The samples used in this study were the pathogenic bacterial isolates BPK-1 and BPK-2 which had previously been isolated from snapper fish originating from Banda Aceh waters. The bacteria were previously grown in nutrient broth (NB) media, then DNA genome from the bacteria was isolated using Presto ™ Mini g DNA Bacteria Kit, followed by PCR amplification of 16S rRNA gene. PCR products were sequenced and nucleotide sequence data were analyzed by bioinformatics software to construct phylogenetic tree. The 16S rRNA gene sequences of the two isolates BPK-1 and BPK-2 were two different bacterial species. BPK-2 isolate has similarities with Aeromonas sp. with a similarity percentage of 96.69%. While the BPK-1 isolate has similarities with Aeromonas salmonicida with a similarity percentage of 85.88%, so it is suspected that it is a novel species that needs further research.

Copyright

© 2021, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Wahyu Eka Sari
AU  - M. Nur Salim
AU  - Teuku Reza Ferasyi
AU  - Muhammad Hambal
AU  - Henni Vanda
AU  - T. Zahrial Helmi
AU  - Mudhita Zikkrullah Ritonga
AU  - Sukmawan Fajar Santosa
AU  - Windy Budi Setiawandi
PY  - 2021
DA  - 2021/04/21
TI  - Identification of Pathogenic Bacteria in Snapper Fish (Lutjanus sp.) from Banda Aceh Waters
BT  - Proceedings of the 2nd International Conference on Veterinary, Animal, and Environmental Sciences (ICVAES 2020)
PB  - Atlantis Press
SP  - 154
EP  - 158
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.210420.033
DO  - 10.2991/absr.k.210420.033
ID  - Sari2021
ER  -