Protein Concentration of Excretory-Secretory and Somatic Antigen of Paramphistomum spp.

DOI

10.2991/absr.k.210420.027How to use a DOI?

Keywords

excretory-secretory antigen, Paramphistomum spp., SDS-PAGE, protein profile, antigen isolation

Abstract

This study was aimed at finding out protein profile of excretory-secretory and somatic antigen of Paramphistomum spp. As many as 60 Paramphistomum spp. taken from cattle in Banda Aceh Municipality Slaughterhouse were placed into 80 ml Roswell Park Memorial Institute (RPMI) 1640 and incubated for 12, 24, 48, and 72 hours. Excretory-secretory as well as somatic antigen was isolated and the concentration of protein was determined using Bradford method, and calibration curve was conducted using Bovine Serum Albumin as standard. To find out the molecular weight of protein, SDS-PAGE method was employed. The linear regression of calibration curve was y= 0.451x + 0.651. The highest concentrations of excretory-secretory antigen protein obtained was 235 ppm, and the somatic protein was 261 ppm. Weight of protein molecules was ranging from 29-130 kDa.

Copyright

© 2021, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Siti Rani Ayuti
AU  - Muhammad Hambal
AU  - Ummu Balqis
AU  - Henni Vanda
PY  - 2021
DA  - 2021/04/21
TI  - Protein Concentration of Excretory-Secretory and Somatic Antigen of Paramphistomum spp.
BT  - Proceedings of the 2nd International Conference on Veterinary, Animal, and Environmental Sciences (ICVAES 2020)
PB  - Atlantis Press
SP  - 128
EP  - 131
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.210420.027
DO  - 10.2991/absr.k.210420.027
ID  - Ayuti2021
ER  -