Liquid Chromatograph – Mass Spectrophotometer and Anti Uric Acid Potential Studies of Ethyl Acetat Extract of Archidendron bubalinum (Jack) I.C. Nielsen Fruit Seed Shell

DOI

10.2991/icst-18.2018.62How to use a DOI?

Keywords

Seed shell of Archidendron bubalinum (Jack) I.C. Nielsen (AbJICN; Liquid Chromatograph Mass Spectrophotometer (LC-MS); allopurinol; 2 4 7- Trinitrofluorenone; anti uric acid

Abstract

This study focused on identifying the types of compounds contained in the ethyl acetate extract of seed shell of Archidendron bubalinum (Jack) I.C. Nielsen (AbJICN ) from Lampung Indonesia using Liquid Chromatograph Mass Spectrophotometer (LC-MS) and determining the potential of anti-uric acid in the identified compounds. The experiment consists of four stages, which are 1) extraction the fruit seed shell of AbJICN using ethyl acetate, 2) phytochemical test, 3) determination of chemical structure using LC-MS and 4) anti uric acid test in vitro by Tri Bromo Hidroksibenzoid Acid (TBHBA) method. The result of phytochemical test found that ethyl acetate extract in fruit seed shell of AbJICN contains compound of flavanoid and terpenoid group. This founding was also supported by the characterization of chemical compounds using LC-MS which indicated the presence of candidates allopurinol and 2 4 7-Trinitrofluorenone compounds. The decreased uric acid by ethyl acetate extract of fruit seed shell was 1.25 mg/dL half times compared to standard allopurinol 2,43 mg/dL. It can be concluded that allopurinol compounds contained in the ethyl acetate extract of seed shell of Archidendron bubalinum (Jack ) I.C. Nielsen potentially became an active anti uric acid substance

Copyright

© 2018, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Erna Styani
AU  - Candra Irawan
AU  - Hanafi Hanafi
AU  - Lilis Sulistiawaty
AU  - Imalia Imalia
PY  - 2018/12
DA  - 2018/12
TI  - Liquid Chromatograph – Mass Spectrophotometer and Anti Uric Acid Potential Studies of Ethyl Acetat Extract of Archidendron bubalinum (Jack) I.C. Nielsen Fruit Seed Shell
BT  - Proceedings of the International Conference on Science and Technology (ICST 2018)
PB  - Atlantis Press
SP  - 293
EP  - 297
SN  - 2589-4943
UR  - https://doi.org/10.2991/icst-18.2018.62
DO  - 10.2991/icst-18.2018.62
ID  - Styani2018/12
ER  -