Physiological and Molecular Characterization of Mutated Spathoglottis plicata

DOI

10.2991/assehr.k.210305.008How to use a DOI?

Keywords

physiological and molecular characteristics, mutant, Spathoglottis plicata

Abstract

A number of Spathoglotis plicata mutant orchids were derived from in vitro culture of X-ray irradiated seeds obtained from previous studies. This study aims to identify the physiological and molecular characteristics of the S. plicata mutant orchid. There were 4 groups of mutant orchids corresponding to the irradiation dose of 6, 12, 18 and 24 rad and 1 wild type group as a control. Observations on physiological variations such as the rate of photosynthesis, chlorophyll content, water conductance, transpiration, total protein and catalase activity were carried out. The chlorophyll content was measured using the Winterman and De Mots method, while the rate of photosynthesis and other related parameters were measured with a Li-Cord-6400 photosynthesis gauge. Molecular characterization was observed from the protein content and catalase activity. Protein content was measured using the Bradford method with bovine serum albumin (BSA) as a protein standard, while the catalytic activity of catalase was determined spectrophotometrically using the Lucks method. The results showed that the photosynthesis rate of mutant orchids tended to be slower but the chlorophyll content, transpiration rate, and water conductance between the mutant group and wild type showed no significant difference. The total protein content and catalytic activity in the two groups also showed no significant difference. These data showed that the mutations have not yet reached a level that affected the expression of genes that control photosynthesis, chlorophyll biosynthesis and catalase.

Copyright

© 2021, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Download article (PDF)

TY  - CONF
AU  - Suyitno Aloysius
AU  - Nur Aeni Ariyanti
AU  - Lili Sugiyarto
AU  - Djukri
PY  - 2021
DA  - 2021/03/08
TI  - Physiological and Molecular Characterization of Mutated Spathoglottis plicata
BT  - Proceedings of the 7th International Conference on Research, Implementation, and Education of Mathematics and Sciences (ICRIEMS 2020)
PB  - Atlantis Press
SP  - 49
EP  - 55
SN  - 2352-5398
UR  - https://doi.org/10.2991/assehr.k.210305.008
DO  - 10.2991/assehr.k.210305.008
ID  - Aloysius2021
ER  -