Effect of Process Parameters on the Production of Cyclodextrin Using Cyclodextrin Glucanotransferase From Bacillus licheniformis

DOI

10.2991/aer.k.201229.004How to use a DOI?

Keywords

Cyclodextrin, cyclodextrin glucanotransferase, pH, temperature, agitation rate

Abstract

Cyclodextrin (CD) is a non-reducing oligosaccharides constituted by variable number of units of glucose. CD has a doughnut-shaped molecule with hydrophilic outer surface and hydrophobic inner cavity. Such structure features enables CD to form inclusion complexes with guest compound. CD is produced by degradation of starch using cyclodextrin glucanotransferase (CGTase). The process parameters such as types of substrate, reaction time and temperature will affect the production of CD. Most of the studies investigated the effect of process parameters using CGTase from Bacillus circulans, Bacillus subtilis and Thermoanaerobacter sp on the CD production. However, no studies have investigated the effect of pH, temperature and agitation rate on the production of CD using CGTase from Bacillus licheniformis. In this present study, the effect of pH (5-9), temperature (20-60°C) and agitation rate (50-250 rpm) of CGTase from Bacillus licheniformis on the CD production were studied. The most suitable pH for the production of CD was pH 6 with 4.45 mg/ml of CD. In addition, the best temperature that produced the highest amount of CD was detected at 40°C with 1.473 mg/ml. The optimal agitation rate was found to be 150 rpm with 2.762 mg/ml of CD. The best process parameters proved to be valuable for the highest production of CD. Hence, the high production of CD may be beneficial for the industrial scale purposes.

Copyright

© 2020, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - R. C. Man
AU  - S. Selvanathan
AU  - S. M. Shaarani
AU  - Z. I. M. Arshad
AU  - S. K. A. Mudalip
AU  - S. Z. Sulaiman
PY  - 2020
DA  - 2020/12/30
TI  - Effect of Process Parameters on the Production of Cyclodextrin Using Cyclodextrin Glucanotransferase From Bacillus licheniformis
BT  - Proceedings of the Third International Conference on Separation Technology 2020 (ICoST 2020)
PB  - Atlantis Press
SP  - 30
EP  - 34
SN  - 2352-5401
UR  - https://doi.org/10.2991/aer.k.201229.004
DO  - 10.2991/aer.k.201229.004
ID  - Man2020
ER  -