Sonication Technique Application on Cellulose Producing Bacteria Acetobacter xylinum

DOI

10.2991/aer.k.201229.006How to use a DOI?

Keywords

Acetobacter xylinum, bacterial cellulose, synthetic medium, sonication

Abstract

Cellulose is abundantly obtained from plant however the main drawback it has to be extracted from its source and purified by chemical separation process to remove the hemicellulose and lignin. Certain bacteria such as Acetobacter xylinum produce cellulose during fermentation process. This technique is more sustainable and the cellulose produced is pure which does not need any purification. Static fermentation methods using synthetic medium Shigeru Yamanaka usually take 14 days to produce bacterial cellulose (BC). Hence research is on-going to improve the fermentation process to be faster, more sustainable and economical. The objective of this study is to investigate the effect of ultrasonic wave on the A. xylinum as well as cellulose production and determine optimum sonication variables for improving yield of BC. Sonication process applies 40 kHz of frequency to the medium culture and glucose was supplied as the carbon source to A. xylinum. By adding ultrasonic wave to culture medium in static method the production of BC was improved with significant increment in yield. The production rate of BC was higher compare to non-sonicated medium however sonication should be at optimum time otherwise the bacterial cell is damaged after too long sonication. In this study the optimum time for sonication was 5 to 7 minutes. Sonication is like imitation of the agitation method but only apply small wave to the medium. It may shows effect on Gram negative bacteria like A. xylinum which have thin peptidoglycan layer. Sonication will enhance the transport process of nutrient in the medium to the bacterial cell.

Copyright

© 2020, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Muhamad Elias Alamin Kamaludin
AU  - Ida Idayu Muhamad
AU  - Saiful Izwan Abd Razak
PY  - 2020
DA  - 2020/12/30
TI  - Sonication Technique Application on Cellulose Producing Bacteria Acetobacter xylinum
BT  - Proceedings of the Third International Conference on Separation Technology 2020 (ICoST 2020)
PB  - Atlantis Press
SP  - 45
EP  - 51
SN  - 2352-5401
UR  - https://doi.org/10.2991/aer.k.201229.006
DO  - 10.2991/aer.k.201229.006
ID  - Kamaludin2020
ER  -