The Effects of Antimicrobial Peptide Gel on Angiogenesis and Fibroblast Cells in Periodontal Tissue Regeneration in a Periodontitis Rats Model Exposed by Nicotine

DOI

10.2991/ahsr.k.210115.036How to use a DOI?

Keywords

angiogenesis, antimicrobial peptide gel, fibroblast, nicotine

Abstract

Nicotine, a major component of tobacco, is a significant contributing factor for the exacerbation of periodontal diseases. Periodontitis, one of the periodontal diseases, leads to alveolar bone damage triggered by bacteria and exacerbated by nicotine exposure as mostly suffered by smokers. Periodontitis treatment requires an antibacterial for the regeneration process; antimicrobial peptide gel (AMP) has a broad-spectrum antimicrobial that is rarely resistant. This study aims to determine the effect of antimicrobial peptide gel on the regeneration process of periodontitis in rat models exposed to nicotine by observing the number of angiogenesis and fibroblast cells. Twenty-four Wistar rats were divided into four groups based on nicotine exposure, the control group and treated rats. Periodontitis was induced by ligation on the mandibular central incisor for 14 days, and the periodontitis rats were treated with AMP on their sulcus of gingiva on day 1, 3, and 7. On day 3, 7, and 14 after treatment, two rats were taken randomly in each group for decapitation, followed by histological processing and examination with Hematoxylin-Eosin. The non-parametric statistic test of Kruskal-Wallis revealed that the value of p=0,000 for angiogenesis and p=0,001 for fibroblast showed significant difference (p <0,05 ) between the treated and control groups in both groups with and without exposure to nicotine. Conclusion: The high number of angiogenesis and fibroblast cells in treated rats indicated that the Antimicrobial Peptide Gel successfully accelerated periodontitis’ regeneration process in rat models exposed to nicotine.

Copyright

© 2021, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Ika Andriani
AU  - Atiek Driana Rahmawati
AU  - Maulida Nurhasanah
AU  - M. Ihza Humanindito
PY  - 2021
DA  - 2021/01/16
TI  - The Effects of Antimicrobial Peptide Gel on Angiogenesis and Fibroblast Cells in Periodontal Tissue Regeneration in a Periodontitis Rats Model Exposed by Nicotine
BT  - Proceedings of the 4th International Conference on Sustainable Innovation 2020–Health Science and Nursing (ICoSIHSN 2020)
PB  - Atlantis Press
SP  - 172
EP  - 176
SN  - 2468-5739
UR  - https://doi.org/10.2991/ahsr.k.210115.036
DO  - 10.2991/ahsr.k.210115.036
ID  - Andriani2021
ER  -