Proceedings of the 4th International Conference on Sustainable Innovation 2020–Health Science and Nursing (ICoSIHSN 2020)

Optimization of Gold Nanoparticles and Molecular Beacon Probe for DNA Detection by Colorimetric Method

Authors
Hari Widada, Mar’atus Sholikhah, Sabtanti Harimurti, Dyani Primasari Sukamdi, Abdul Rohman, Riris Istighfari Jenie, Sismindari
Corresponding Author
Hari Widada
Available Online 16 January 2021.
DOI
10.2991/ahsr.k.210115.081How to use a DOI?
Keywords
Biosensor, Colorimetry, Gold Nanoparticles, Molecular Beacon
Abstract

Gold nanoparticles are platforms that can be immobilized with a probe; thus, they can be used as biosensors. In the health sector, biosensors are utilized for biomedical applications, such as for DNA marking and cell isolation. In addition, it can be used as a method for Halal Authentication applications for meat products. This study aims to determine the optimum conditions for the synthesis of gold nanoparticles as a platform for immobilization and application of AuNPs-MB as biosensors with the colorimetry method for Halal Authentication applications of meat product. This research optimized gold nanoparticles using 6 series of HAuCl4 concentration, namely 0.0635; 0.127; 0.19; 0.254; 0.635; dan 1.27 (mM). The most optimum concentration was immobilized with probe MB and measured using Uv-Vis Spectrophotometer to identify the absorption and Scanning Electron Microscope (SEM) to identify nanoparticles’ size distribution. The results showed that 0.19 mM of AuNPs gave the best results, which was stable for 3.5 months and had good results measured using SEM with an average size of 21.7 nm. The value of absorbance, before immobilization, decreased from 0.226 to 0.167 and indicated that the prose was successful. Based on the result of this study, it can be concluded that AuNPs-MB was proven to be a biosensor for Halal Authentication applications using the colorimetric method.

Copyright
© 2021, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 4th International Conference on Sustainable Innovation 2020–Health Science and Nursing (ICoSIHSN 2020)
Series
Advances in Health Sciences Research
Publication Date
16 January 2021
ISBN
978-94-6239-315-8
ISSN
2468-5739
DOI
10.2991/ahsr.k.210115.081How to use a DOI?
Copyright
© 2021, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Hari Widada
AU  - Mar’atus Sholikhah
AU  - Sabtanti Harimurti
AU  - Dyani Primasari Sukamdi
AU  - Abdul Rohman
AU  - Riris Istighfari Jenie
AU  - Sismindari
PY  - 2021
DA  - 2021/01/16
TI  - Optimization of Gold Nanoparticles and Molecular Beacon Probe for DNA Detection by Colorimetric Method
BT  - Proceedings of the 4th International Conference on Sustainable Innovation 2020–Health Science and Nursing (ICoSIHSN 2020)
PB  - Atlantis Press
SP  - 408
EP  - 411
SN  - 2468-5739
UR  - https://doi.org/10.2991/ahsr.k.210115.081
DO  - 10.2991/ahsr.k.210115.081
ID  - Widada2021
ER  -