Proceedings of the 1st International Conference on Industry Science Technology and Sustainability (IConISTS 2023)

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Optimizing Lipid Content and Biomass Production with Combined EMS and UV-C Treatment on 3 Species of Microalgae

Authors
Elida Purba1, *, Kanesia Natalie Yobel1
1Center of Green and Clean Energy, Department of Chemical Engineering, University of Lampung, Bandar Lampung, Indonesia, 35145
*Corresponding author. Email: elida.purba@eng.unila.ac.id
Corresponding Author
Elida Purba
Available Online 2 August 2024.
DOI
10.2991/978-94-6463-475-4_33How to use a DOI?
Keywords
Microalgae; Lipid content; EMS Mutagen; Biomass productivity; Photobioreactor; Cell density
Abstract

This paper elaborates about the enhancing of lipid content and biomass productivity of the three types of microalgae. The aim of this study is to determine the effect of EMS (ethyl methanesulfonate) mutagen and UV-C on microalgae Spirulina sp., Dunaliella sp., and Nitzschia sp., and comparing their biomass and lipid productivity. Both treatments by EMS mutated microalgae and UV-C light were applied to both wild-type and mutated microalgae. The cultivation was carried out for 7 days in a photobioreactor with a capacity of 8 l. The treatment for mutated microalgae was carried out by adding 0.5 M EMS and UV-C irradiation for 3 minutes. Cell density was calculated from day 1 to 7 on each type of microalgae using a haemocytometer. The results showed that mutagens cause an increase in cell density in Dunaliella sp. up to 5.7% and 6.5%. increase in biomass. In Nitzschia sp. there was also an increase in cell density up to 16.5% and an increase in biomass of 25.3% from the wild type, however, there was a decrease in cell density and biomass productivity in Spirulina sp. Mutagens also affects the lipid content of microalgae because it has a significant effect on increasing the lipid content of mutated microalgae by 1.93-fold in Spirulina sp., 0.08-fold in Dunaliella sp., and 0.44-fold in Nitzschia sp.

Copyright
© 2024 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the 1st International Conference on Industry Science Technology and Sustainability (IConISTS 2023)
Series
Advances in Engineering Research
Publication Date
2 August 2024
ISBN
10.2991/978-94-6463-475-4_33
ISSN
2352-5401
DOI
10.2991/978-94-6463-475-4_33How to use a DOI?
Copyright
© 2024 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

risenwbib
TY  - CONF
AU  - Elida Purba
AU  - Kanesia Natalie Yobel
PY  - 2024
DA  - 2024/08/02
TI  - Optimizing Lipid Content and Biomass Production with Combined EMS and UV-C Treatment on 3 Species of Microalgae
BT  - Proceedings of the 1st International Conference on Industry Science Technology and Sustainability (IConISTS 2023)
PB  - Atlantis Press
SP  - 389
EP  - 405
SN  - 2352-5401
UR  - https://doi.org/10.2991/978-94-6463-475-4_33
DO  - 10.2991/978-94-6463-475-4_33
ID  - Purba2024
ER  -