The Comparison of Catechol-o-methyltransferase VAL158MET on Schizophrenic Bataknese Patients

DOI

10.2991/978-94-6463-310-8_6How to use a DOI?

Keywords

Catechol O-Methyltransferase; Indonesia; Polymorphism; Single Nucleotide

Abstract

Background

Schizophrenia is a mental disorder that is common throughout the world. Schizophrenia is estimated to occur in 24 million people or one in 3000 people (0.32%). In Indonesia, it is estimated that around 6.9% of family members have schizophrenia. Genetic and epidemiological studies have linked environmental factors to genetic factors, including place of birth, infections, complications during pregnancy and delivery, effects of medications, and stress. From a genetics perspective, one hypothesis is through catechol-O-methyltransferase (COMT) polymorphism, where there are two forms, namely the soluble form (S form) and the membrane-bound form (M form) located on chromosome 22q11. The COMT gene polymorphism is a single nucleotide polymorphism (SNP) rs4680, which can change Val to Met at codon 158 (Val158Met), which occurs from the M form of the gene. This gene polymorphism is widely considered to have a strong relationship with schizophrenia.

Methods

This is an analytical comparative study with a cross-sectional design, in which 400 samples were recruited into case groups of Bataknese people with schizophrenia who have been hospitalized in the installation unit in North Sumatera Mental Hospital and a control group of blood-donors members at North Sumatera Red Cross. The study was carried out from October 2022 to March 2023. Blood sample collection was carried out via consecutive nonprobability sampling type, while Polymerase Chain Reaction – Restriction Fragment Length Polymorphism (PCR-RFLP) on the examination of COMT polymorphisms was performed.

Results

The appearance of Val alleles (N=351 times) was found more in control groups than in the cases group, while the Met alleles appeared 89 times in the PWS groups. The genotype variations of ValVal were found to be the highest variant in Batak PWS groups for 123 (61.5%), and interestingly, there were similar variants of ValMet in both groups, accounting for 34.0%. In comparison, the genotype of ValVal and MetMet had a p-value of 0.013, with OR=0.232, CI 95% between 0.085-0.633. Meanwhile, the comparison of ValMet and MetMet showed a p=0.808, OR=0.271, with CI between 0.097-0.760.

Conclusion

Individuals with Val alleles display possible mechanisms to develop schizophrenia compared to those with Met alleles. As far as the genotype of Val158Met is concerned towards the risk factors of developing schizophrenia, no significant difference was found between the Batak PWS groups and the control group in terms of genotype appearance.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - David Erickson Simanjuntak
AU  - Elmeida Effendy
AU  - Mustafa M. Amin
PY  - 2023
DA  - 2023/12/18
TI  - The Comparison of Catechol-o-methyltransferase VAL158MET on Schizophrenic Bataknese Patients
BT  - Proceedings of the 6th International Conference of Neuroscience, Neurology and Psychiatry Universitas Sumatera Utara (ICONAP 2023)
PB  - Atlantis Press
SP  - 47
EP  - 52
SN  - 2468-5739
UR  - https://doi.org/10.2991/978-94-6463-310-8_6
DO  - 10.2991/978-94-6463-310-8_6
ID  - Simanjuntak2023
ER  -