The Effectiveness of Lerak Fruit (Sapindus Rarak DC) As an Alternative to Xylol in the Hematoxylin-Eosin Staining Deparaffinization Process

DOI

10.2991/978-94-6463-324-5_45How to use a DOI?

Keywords

Deparaffinization; Xylol; Lerak

Abstract

Deparaffinization is the initial stage in the staining process which aims to remove paraffin from the tissue so that it can absorb the dye properly. The solution used at this stage is xylol because it is non-polar. However, in its use xylol has disadvantages, one of which is toxic. Lerak contains saponin compounds. Saponin in lerak can function as a surfactant so that it can dissolve paraffin. The purpose of this study was to observe the quality of restosigmoid, mammary, endometrial, uterine, renal, lymph node, and uterine leiomyoma tissue preparations, which were deparaffinized using xylol and lerak. This study was conducted at the Anatomical Pathology Laboratory of Dr. Soetomo Hospital Surabaya in March - May 2023. The type of research used was laboratory experimental with post-test and control group design. The samples used were 144 preparations from 6 different tissues deparaffinized with xylol and 1%, 3%, 5%, 7%, & 9% lerak solution. The results of preparations deparaffinized with xylol based on the Kruskal wallis test showed an average (mean rank = 22.50). The results of preparations deparaffinized with lerak based on the Kruskal wallis test showed that the highest mean rank value was occupied by 1% lerak with an average (mean rank = 18.50). If based on the type of tissue, 5% lerak is effective as an alternative to xylol in lymph node tissue. It was concluded that lerak is not yet effective as an alternative to xylol in the deparaffinization process.

Copyright

© 2023 The Author(s)

Open Access

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TY  - CONF
AU  - Erlin Walangara
AU  - Lully Hanni Endarini
AU  - Anik Handayati
AU  - Evy Diah Woelan Sari
AU  - Wisnu Istanto
PY  - 2023
DA  - 2023/12/23
TI  - The Effectiveness of Lerak Fruit (Sapindus Rarak DC) As an Alternative to Xylol in the Hematoxylin-Eosin Staining Deparaffinization Process
BT  - Proceedings of the 6th International Conference of Health Polytechnic Surabaya (ICoHPS 2023)
PB  - Atlantis Press
SP  - 448
EP  - 455
SN  - 2468-5739
UR  - https://doi.org/10.2991/978-94-6463-324-5_45
DO  - 10.2991/978-94-6463-324-5_45
ID  - Walangara2023
ER  -