Proceedings of the 3rd International Conference on Biology, Science and Education (IcoBioSE 2021)

The Effect of Administration of Honey on Maximal Physical Activity in Malondialdehyd (Mda) Levels of Male Mice (Mus musculus L.)

Authors
Pudia M. Indika1, *, Randi Kurniawan1, Ridho Bahtra1, Elsa Yuniarti2
1Sport Science Faculty, Universitas Negeri Padang, Padang, Indonesia
2Biology Department, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang, Padang, Indonesia
*Corresponding author. Email: pudia_dr@fik.unp.ac.id
Corresponding Author
Pudia M. Indika
Available Online 22 May 2023.
DOI
10.2991/978-94-6463-166-1_25How to use a DOI?
Keywords
Malondialdehyde; Maximal physical activity; Honey
Abstract

Maximum physical activity creates an imbalance between the production of free radicals and the body’s antioxidant defense system known as oxidative stress. Maximum physical activity increases the need for oxygen, which leads to an increase in reactive oxygen species (ROS) and free radical products. Thus causing increased levels of malondialdehyde (MDA), this can be overcome by consuming honey which contains antioxidants. The purpose of this study was to determine the effect of honey administration on maximal physical activity of MDA levels in male mice (Mus musculus L.). The research used is experimental in vivo laboratory which was selected because both the sample and the treatment are more controlled, and measurable, and the effect of the treatment can be trusted with a Completely Randomized Design (CRD). Mice were then divided into 4 groups, namely K− (negative control), K+ (positive control), P1 (giving 0.5 ml of honey before maximum physical activity), and P2 (giving 1 ml of honey before maximum physical activity) (honey by a dosage of 100 ml) mg/kg BW which was induced with a volume of 0.5 ml and 1 ml. Each group consisted of 7 mice and was subsequently treated. The examination was carried out in the form of MDA levels of male mice using the MDA Kit PP. The maximum physical activity treatment was swimming and giving honey for 7 days which had previously been acclimatized for 7 days. The data were analyzed in one-way variance (One Way ANOVA) followed by a further LSD test with a level of 5%. The results of this study showed that feeding honey was effective (p < 0.05) in reducing the amount of malondialdehyde (MDA) levels in male mice by 2.68 nmol/ml. The most effective administration of honey was at the highest dose of P2 (concentration 100 mg, dose 1 mL) with a malondialdehyde 2.87 nmol/ml. This research was conducted as an effort to provide the ideal dose for mice so that the dose of honey used could be converted to human needs as a recommendation for providing honey in the area of physical activity.

Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the 3rd International Conference on Biology, Science and Education (IcoBioSE 2021)
Series
Advances in Biological Sciences Research
Publication Date
22 May 2023
ISBN
978-94-6463-166-1
ISSN
2468-5747
DOI
10.2991/978-94-6463-166-1_25How to use a DOI?
Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

TY  - CONF
AU  - Pudia M. Indika
AU  - Randi Kurniawan
AU  - Ridho Bahtra
AU  - Elsa Yuniarti
PY  - 2023
DA  - 2023/05/22
TI  - The Effect of Administration of Honey on Maximal Physical Activity in Malondialdehyd (Mda) Levels of Male Mice (Mus musculus L.)
BT  - Proceedings of the 3rd International Conference on Biology, Science and Education (IcoBioSE 2021)
PB  - Atlantis Press
SP  - 171
EP  - 180
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-166-1_25
DO  - 10.2991/978-94-6463-166-1_25
ID  - Indika2023
ER  -