Study on Fluorescence Spectra of B Vitamins

DOI

10.2991/icmmse-16.2016.15How to use a DOI?

Keywords

Thiamine/Vitamin B1, Riboflavin/Vitamin B2; Pyridoxine/Vitamin B6, Fluorescence Spectra, Compound Vitamin B, PARAFAC

Abstract

This paper presents the intrinsic fluorescence characteristics of vitamin B1, B2 and B6 measured with 3D fluorescence Spectrophotometer. Three strong fluorescence areas of vitamin B2 locate at ?ex/?em=270/525nm, 370/525nm and 450/525nm, one fluorescence area of vitamin B1 locates at ?ex/?em=370/460nm, two fluorescence areas of vitamin B6 locate at ?ex/?em=250/370nm and 325/370nm were found. Using the PARAFAC algorithm, 10 vitamin B1, B2 and B6 mixed solutions were successfully decomposed, the emission profiles, excitation profiles, central wavelengths and the concentration of the three components were retrieved precisely through about 5 iteration times. Vitamin B1, B2 and B6 components were found in two compound vitamin B samples, the fluorescence contribution of pyridoxine is weakest in both samples, and the concentration of riboflavin, and thiamine are 0.11mg/l, 0.32mg/l respectively for sample 1st, and 0.12mg/L for sample 2nd.

Copyright

© 2016, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Xiao Xue
AU  - Lan Hu
AU  - Zhibin Hou
PY  - 2016/03
DA  - 2016/03
TI  - Study on Fluorescence Spectra of B Vitamins
BT  - Proceedings of the 2016 International Conference on Mechanics, Materials and Structural Engineering
PB  - Atlantis Press
SP  - 85
EP  - 90
SN  - 2352-5401
UR  - https://doi.org/10.2991/icmmse-16.2016.15
DO  - 10.2991/icmmse-16.2016.15
ID  - Xue2016/03
ER  -