Facile construction of novel organic-inorganic hybrid microspheres for enzyme immobilization

DOI

10.2991/icmemtc-16.2016.45How to use a DOI?

Keywords

Membrane emulsification; Biomimetic mineralization; Enzyme immobilization.

Abstract

In this research, a novel and facile method for preparing organic-inorganic hybrid microspheres was developed by a synergy of membrane emulsification and biomimetic mineralization. The gelatin solution (water phase) was extruded through a Shirasu Porous Glass (SPG) membrane, and mixed with the liquid paraffin (oil phase) to form a monodispersed W/O emulsion. After cooling and cross-linking, the organic gelatin microspheres were obtained, and then immersed in to Na2SiO3 solution, which acted as silicon precursor. Under the catalysis of gelatin, silica coating was formed around the gelatin microspheres, resulting in the final gelatin/silica hybrid microspheres. The as-prepared hybrid microspheres were subsequently utilized for encapsulation of -Glucuronidase (GUS). The results indicated that the recycling and storage stability of the immobilized enzyme are all significantly improved.

Copyright

© 2016, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Jun Ma
AU  - Shuo Liu
AU  - Yanhui Chen
AU  - Jian Li
PY  - 2016/04
DA  - 2016/04
TI  - Facile construction of novel organic-inorganic hybrid microspheres for enzyme immobilization
BT  - Proceedings of the 2016 3rd International Conference on Materials Engineering, Manufacturing Technology and Control
PB  - Atlantis Press
SP  - 235
EP  - 239
SN  - 2352-5401
UR  - https://doi.org/10.2991/icmemtc-16.2016.45
DO  - 10.2991/icmemtc-16.2016.45
ID  - Ma2016/04
ER  -