Down-regulation of MEF2D via HBc Nanoparticle Mediated siRNA Inhibits HepG2 Cell Proliferation in vitro
- DOI
- 10.2991/icimm-15.2015.44How to use a DOI?
- Keywords
- MEF2D; Hepatoma Cells; Proliferation; Gene Therapy; HBc Particles
- Abstract
Objective: Recombinant hepatitis B virus nucleocapsid protein maintains the ability to form particles self-assembly. We tried to use non-virus-like particles HBc package wrapping foreign gene to protect and transfer siRNA of MEF2D gene to target position, in order to make a contribution to analyze the relationship between the expression of MEF2D gene and the proliferation of hepatocellular cells. Methods: Firstly, prepared si-MEF2D/HBc nanoparticles and transfected it into hepatocellular cells. The knockdown of MEF2D gene effects on cell proliferation was detected by MTT assay, XTT cell viability assay and CKK-8 assays. Result: The proliferation of treated hepatocellular cells were decreased significantly compared with control cells. Pretreatment of HepG2 cells with si-MEF2D/HBc complex inhibited cell growth in a dose-dependent manner. Conclusion: HBc particles is one kind of promising biological nanomaterials which has presented itself as an appropriate and valuable material for other aspects of nanoparticle in future study.
- Copyright
- © 2015, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Jing Kong AU - Xiaoping Liu AU - Jun Chen AU - Fang Fang PY - 2015/07 DA - 2015/07 TI - Down-regulation of MEF2D via HBc Nanoparticle Mediated siRNA Inhibits HepG2 Cell Proliferation in vitro BT - Proceedings of the 5th International Conference on Information Engineering for Mechanics and Materials PB - Atlantis Press SP - 230 EP - 235 SN - 2352-5401 UR - https://doi.org/10.2991/icimm-15.2015.44 DO - 10.2991/icimm-15.2015.44 ID - Kong2015/07 ER -