Antioxidant and Anti-aging Activity of Parijoto Fruit (Medinilla Speciosa, Reinw.ex Bl.) Ethanolic Extract In Vitro

DOI

10.2991/978-94-6463-431-0_5How to use a DOI?

Keywords

Anti-aging; Antioxidant; Elastase enzyme; Fibroblast cell; Parijoto (Medinilla speciosa)

Abstract

The Human skin can experience physiological changes with age, resulting in skin aging. There are several types of factors that cause skin aging, such as intrinsic and extrinsic factors. Parijoto fruit (Medinilla speciosa, Reinw.ex Bl) contains polyphenolic compounds that can improve the balance of ROS (Reactive Oxygen Species) with a mechanism as a free radical inhibitor. Avoid symbols and abbreviations in the abstract. This study aims to determine the biological activity of Medinilla speciosa, Reinw.ex Bl fruit as an anti-aging active ingredient in vitro. The parijoto fruit powder was macerated using 70% ethanol followed by phytochemicals screening. Antioxidant activity was tested using DPPH method (quercetin as a positive control). The in vitro anti-aging activity was tested using MTT Assay on Human Dermal Fibroblast adult (HDFa), meanwhile the elastase enzyme inhibition test was conducted using Human Neutrophil Elastase (HNE). The results showed that parijoto fruits extract contained saponins, tannins, and flavonoids. The activity of parijoto fruits extract with an IC₅₀ value of 122.19 g/mL was categorized as a medium antioxidant. Parijoto fruit extract was proven to inhibit elastase enzyme activity with a percentage of inhibition at 50 μg/mL, 100 μg/mL, and 200 μg/mL concentrations is 80%, 80%, and 60%, respectively. The HDFa cell viability test showed that ethanol extract of parijoto fruit could affect maintained HDFa cell viability against exposure to H₂O₂ at a concentration of 31.25;250;500;1000 μg/mL. Based on the results, the ethanolic extract of parijoto fruit possesses anti-aging activity.

Copyright

© 2024 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Aji Winanta
AU  - Gian Artika Sari
AU  - Rifki Febriansah
AU  - Arde Toga Nugraha
PY  - 2024
DA  - 2024/05/30
TI  - Antioxidant and Anti-aging Activity of Parijoto Fruit (Medinilla Speciosa, Reinw.ex Bl.) Ethanolic Extract In Vitro
BT  - Proceedings of selected papers of International Conference on Health, Science, and Environment (ICHSE 2023)
PB  - Atlantis Press
SP  - 35
EP  - 45
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-431-0_5
DO  - 10.2991/978-94-6463-431-0_5
ID  - Winanta2024
ER  -