The Experimental Study of the Influence of Yanlingdan Prescription on the Apoptosis Protein Fas/FasL Expression of Ovarian Granular Cells in Mice with Premature Ovarian Failure

DOI

10.2991/icemc-17.2017.49How to use a DOI?

Keywords

Yanlingdan prescription; Granular cells; Apoptosis proteins; FAS; FASL

Abstract

Objective: The purpose of this research is to observe the influence of Yanlingdan prescription on the apoptosis protein Fas/FasL expression of ovarian granular cells in mice with autoimmune premature ovarian failure and investigate the mechanism of this prescription in the treatment of premature ovarian failure. Method: Taking the Zona pellucida3 of mice as the antigen, the female mice were injected subcutaneously with this drug to make mice immune so as to become the mice with premature ovarian failure. Blank control group, model group, Yanlingdan group and western medicine group are set. After two weeks of dosing mice, we use immunohistochemical method to detect the expression level of apoptosis related protein Fas/FasL of ovarian granular cells. Results:The expression level of apoptosis related protein Fas/FasL in model group is obviously higher than the blank control group (P<0.01). Compared with model group, the FAS and FASL protein expression of mice' follicle in Yanlingdan group and western medicine group weakens obviously (P<0.01). When the expression of Fas and Fasl in Yanlingdan group compares with the normal control group, the difference has no statistical significance (P>0.05). Conclusion: Yanlingdan prescription can effectively suppress the expression of FAS/FASL protein of follicular granular cells and have inhibitory effects on the apoptosis of ovarian granular cells in mice with premature ovarian failure.

Copyright

© 2017, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Wang Yang
AU  - Li PengHui
AU  - Li CuiPing
AU  - Wang Bin
AU  - Niu ShuDong
AU  - Wen LiBo
AU  - Zhao Kun
AU  - Wang Yuge
AU  - Wang Yuefei
PY  - 2016/06
DA  - 2016/06
TI  - The Experimental Study of the Influence of Yanlingdan Prescription on the Apoptosis Protein Fas/FasL Expression of Ovarian Granular Cells in Mice with Premature Ovarian Failure
BT  - Proceedings of the 7th International Conference on Education, Management, Information and Computer Science (ICEMC 2017)
PB  - Atlantis Press
SP  - 246
EP  - 249
SN  - 2352-538X
UR  - https://doi.org/10.2991/icemc-17.2017.49
DO  - 10.2991/icemc-17.2017.49
ID  - Yang2016/06
ER  -