Proceedings of the 2018 7th International Conference on Energy, Environment and Sustainable Development (ICEESD 2018)

Molecular Biological Identification and Determination of Growth Conditions of Facultative CO2 and N2 Fixing Bacterium BHJ

Authors
Sheng Zhou, Xiaoming Wang
Corresponding Author
Sheng Zhou
Available Online May 2018.
DOI
10.2991/iceesd-18.2018.128How to use a DOI?
Keywords
facultative CO2 and N2 fixing bacterium, carbon- and nitrogen-free medium, molecular biology identification, optimum growth conditions.
Abstract

Currently, the greenhouse effect is becoming increasingly serious, and biological carbon-fixation, especially microbial biological carbon-fixation, can play a unique role in curbing the greenhouse effect.CO2 and N2 are the major greenhouse gases. In this study, a facultative CO2 and N2 fixing bacterium named BHJ (which can fix CO2 and N2 simultaneously) was identified using molecular biology, and its growth conditions were determined. Total DNA from BHJ was extracted and then the 16S rDNA was amplified using polymerase chain reaction. The 16S rDNA sequence was identified and analyzed by alignment with sequences in the GenBank database. Single variables in medium without carbon and nitrogen (KH2PO4 0.2 g, CaCl2 formula: 0.2 g, NaCl 0.2 g, MgSO4 0.3 g, VB 0.0625 g, trace element solution 0.5 mL, distilled water: 1000 mL) was performed. Spectrophotometry was used to measure the optical density changes to determine the optimum temperature, CO2 and N2 ratio, and pH for the growth of BHJ. The 16S rDNA sequence of BHJ showed more than 99% homology with that of Burkholderia cepacia. For the growth of BHJ, the optimum temperature was 34C, the best concentration ratio of CO2 and N2 was 1:99, and the optimum pH was 5.

Copyright
© 2018, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 2018 7th International Conference on Energy, Environment and Sustainable Development (ICEESD 2018)
Series
Advances in Engineering Research
Publication Date
May 2018
ISBN
978-94-6252-503-0
ISSN
2352-5401
DOI
10.2991/iceesd-18.2018.128How to use a DOI?
Copyright
© 2018, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Sheng Zhou
AU  - Xiaoming Wang
PY  - 2018/05
DA  - 2018/05
TI  - Molecular Biological Identification and Determination of Growth Conditions of Facultative CO2 and N2 Fixing Bacterium BHJ
BT  - Proceedings of the 2018 7th International Conference on Energy, Environment and Sustainable Development (ICEESD 2018)
PB  - Atlantis Press
SP  - 695
EP  - 700
SN  - 2352-5401
UR  - https://doi.org/10.2991/iceesd-18.2018.128
DO  - 10.2991/iceesd-18.2018.128
ID  - Zhou2018/05
ER  -