Screening and Fermentation of Jinggang-mycin High-yielding Strains

DOI

10.2991/iceep-18.2018.90How to use a DOI?

Keywords

Jinggang-mycin; compound mutagenic; protoplast; resistance screening

Abstract

Using KN-16 as original strains, adopting compound mutagenic of ultraviolet irradiation, 60Coγ-ray irradiation, protoplast ultraviolet irradiation, measuring the content of Jinggang-mycin A by gas chromatography. The mutagenesis condition was determined by lethality testing, flask fermentation, resistance screening of Jinggang-mycin A. When ultraviolet irradiation time at 9s, 60Coγ-ray dose at 300 Gy, we got strains of K-108, prepared the protoplast, ultraviolet irradiation time at 15s， and screening of tolerated maximum Jinggang-mycin A concentration of 5.5g/100mL, We got stable high-yield strains of 2-27-1, its Jinggang-mycin A concentration in biomass was 4.48g/100mL. Compared with the original strains, the production increased 134.6%. For the mutant strains in this study, the colony morphology is irregular, yellow, light-colored, and no obvious intermediate water absorbent, the growth of colonies in the resistance plate is small, this is different to traditional view.

Copyright

© 2018, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Xiaoju Zhang
AU  - Shijie Li
AU  - Jiangrong Xiao
PY  - 2018/09
DA  - 2018/09
TI  - Screening and Fermentation of Jinggang-mycin High-yielding Strains
BT  - Proceedings of the 2018 7th International Conference on Energy and Environmental Protection (ICEEP 2018)
PB  - Atlantis Press
SP  - 512
EP  - 516
SN  - 2352-5401
UR  - https://doi.org/10.2991/iceep-18.2018.90
DO  - 10.2991/iceep-18.2018.90
ID  - Zhang2018/09
ER  -