A general workflow for differential expression analysis of RNA-seq and introductions on related tools

DOI

10.2991/iceeecs-16.2016.69How to use a DOI?

Keywords

RNA-seq, differential expression analysis, TopHat2, Cufflinks

Abstract

RNA-seq technology have been used widely in many biological research field, but analyzing the enormous RNA-seq data is still a big challenge to biologist without sufficient knowledge of bioinformatics. Considering that the differential expression analysis is a crucial and tricky application of RNA-seq, we introduce a general workflow in this paper. In order to make our instruction more practical, we also provide the basic usage of some famous analysis tools for each step of the workflow.

Copyright

© 2016, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Zhong Zhang
PY  - 2016/12
DA  - 2016/12
TI  - A general workflow for differential expression analysis of RNA-seq and introductions on related tools
BT  - Proceedings of the 2016 4th International Conference on Electrical & Electronics Engineering and Computer Science (ICEEECS 2016)
PB  - Atlantis Press
SP  - 328
EP  - 338
SN  - 2352-538X
UR  - https://doi.org/10.2991/iceeecs-16.2016.69
DO  - 10.2991/iceeecs-16.2016.69
ID  - Zhang2016/12
ER  -