Proceedings of the 2016 International Conference on Civil, Transportation and Environment

Determination of Serum Albumin by its quenching effect on the fluorescence of Zn2+ -Morin complex

Authors
Wei Huang, Nan Cao, Feng Wang
Corresponding Author
Wei Huang
Available Online January 2016.
DOI
10.2991/iccte-16.2016.126How to use a DOI?
Keywords
Morin, BSA,Zn2 +,fluorescence spectrum,UV absorption spectra.
Abstract

In this paper, the mode of action of Morin and protein has been studied through UV and fluorescence spectrum. This paper found that in the pH = 8.50 Tris-HCl buffer solution,at the presence of Zn2+, the protein can cause Morin fluorescence quenching and the quenching degree of fluorescence is in proportional to the amount of protein to a certain extent. At the best condition, the detection limit of BSA and HSA is 0.22 g•mL-1 0.18 g•mL-1 respectively. Compared with other analysis methods for proteins that have been reported,this method has a relatively wide linear range and high sensitivity.

Copyright
© 2016, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Download article (PDF)

Volume Title
Proceedings of the 2016 International Conference on Civil, Transportation and Environment
Series
Advances in Engineering Research
Publication Date
January 2016
ISBN
978-94-6252-185-8
ISSN
2352-5401
DOI
10.2991/iccte-16.2016.126How to use a DOI?
Copyright
© 2016, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Wei Huang
AU  - Nan Cao
AU  - Feng Wang
PY  - 2016/01
DA  - 2016/01
TI  - Determination of Serum Albumin by its quenching effect on the fluorescence of Zn2+ -Morin complex
BT  - Proceedings of the 2016 International Conference on Civil, Transportation and Environment
PB  - Atlantis Press
SP  - 743
EP  - 746
SN  - 2352-5401
UR  - https://doi.org/10.2991/iccte-16.2016.126
DO  - 10.2991/iccte-16.2016.126
ID  - Huang2016/01
ER  -