The Cytotoxic Effect of Ethanolic Extract of Dasun Tunggal and Garlic (Allium sativum) on Raw 264.7 Cells
- DOI
- 10.2991/ahsr.k.211105.005How to use a DOI?
- Keywords
- dasun tunggal; garlic; Allium sativum; MTT assay; raw 264; 7 cells
- Abstract
Traditionally, dasun tunggal and garlic (Allium sativum) have been widely used as immunomodulators. Therefore, scientific research is needed to support this traditional use. The first step that needs to be done is to see the cytotoxic effect of the ethanol extract of dasun tunggal and garlic on raw 264.7 cells. The method used is the MTT method (3-(4,5,-dimethylthiazol-2yl-2,5- diphenyl-tetrazolium bromide). Raw 264.7 cells were cultured on 96 well plates treated with ethanol extract of dasun tunggal and garlic with concentrations of 100, 10, 1 and 0.1 μg/ml. Cells were incubated for 48 hours at 37°C, 5% CO2 and then given 100 μl MTT solution 0,5 mg/ml in PBS (Phosphate Buffer Saline) for 4 hours. Formazan crystals formed were dissolved with 100 μl DMSO and absorbency was measured using a microplate reader. Cell viability was obtained in the dasun tunggal ethanol extract group, respectively, at concentrations of 100; 10; 1 and 0.1 μg/ml were 103.9, 107.6, 114.2, 122.5 % and in the garlic ethanol extract group 124.2, 122.5, 123.6, 121.7 %. The results in both groups were >100%, meaning that the ethanol extract of dasun tunggal and garlic does not have a cytotoxic effect on raw 264.7 cells.
- Copyright
- © 2021 The Authors. Published by Atlantis Press International B.V.
- Open Access
- This is an open access article under the CC BY-NC license.
Cite this article
TY - CONF AU - Irene Puspa Dewi AU - Fatma Sri Wahyuni AU - Yufri Aldi AU - Dachriyanus PY - 2021 DA - 2021/11/17 TI - The Cytotoxic Effect of Ethanolic Extract of Dasun Tunggal and Garlic (Allium sativum) on Raw 264.7 Cells BT - Proceedings of the 2nd International Conference on Contemporary Science and Clinical Pharmacy 2021 (ICCSCP 2021) PB - Atlantis Press SP - 30 EP - 34 SN - 2468-5739 UR - https://doi.org/10.2991/ahsr.k.211105.005 DO - 10.2991/ahsr.k.211105.005 ID - Dewi2021 ER -