Total Phenolic, Total Flavonoid Content, and α-Glucosidase Inhibitory Activity of Centella asiatica (L.) Urb.

DOI

10.2991/ahsr.k.211105.033How to use a DOI?

Keywords

Diabetes mellitus; Centella asiatica (L.) Urb.; total phenolic; flavonoid content; α-glucosidase inhibitory activity

Abstract

Public awareness about the side effects of chemical drugs has increased in recent years. This has resulted in the rapid use of natural ingredients as medicine. Diabetes mellitus is one of the leading causes of death in the world. A large number of people with diabetes and the high level of need for drugs encourage efforts to find new sources of drugs. Centella asiatica (L.) Urb. has been widely used in folk medicine to treat several ailments, including antimicrobial, antioxidant, and anti-inflammation. However, the potential anti-diabetic activity of C. asiatica still needs to be explored. This present study aims to investigate the anti-diabetic potential of the aerial parts of C. asiatica (L.) Urb. by determining total phenolic and flavonoid content, also α-glucosidase inhibitory activity from ethanol extract, n-hexane, ethyl acetate, and water fractions. Bioassay-guided fractionation has been carried out to identify the bioactive crude fractions responsible for α-glucosidase inhibitory activity using in vitro study. The results showed that ethyl acetate fraction of C. asiatica afforded the highest total phenolic and flavonoid content by 14.41 ± 0.020 mg GAE/g extract and 48.43 ± 0.052 mg QE/g extract, respectively. In addition, this fraction also demonstrated the highest α-glucosidase inhibitory activity with IC₅₀ 47.52 ± 0.030 μg/mL. The results indicated that C. asiatica has a potential source as a natural anti-diabetic agent.

Copyright

© 2021 The Authors. Published by Atlantis Press International B.V.

Open Access

This is an open access article under the CC BY-NC license.

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TY  - CONF
AU  - Minarti
AU  - Megawati
AU  - Sofa Fajriah
PY  - 2021
DA  - 2021/11/17
TI  - Total Phenolic, Total Flavonoid Content, and α-Glucosidase Inhibitory Activity of Centella asiatica (L.) Urb.
BT  - Proceedings of the 2nd International Conference on Contemporary Science and Clinical Pharmacy 2021 (ICCSCP 2021)
PB  - Atlantis Press
SP  - 222
EP  - 226
SN  - 2468-5739
UR  - https://doi.org/10.2991/ahsr.k.211105.033
DO  - 10.2991/ahsr.k.211105.033
ID  - 2021
ER  -